Dissertation > Excellent graduate degree dissertation topics show

Screening of Age-related DNA Methylation Fragments

Author: XuLongChang
Tutor: HuangDaiXin
School: Huazhong University of Science and Technology
Course: Forensic Biology
Keywords: Forensic genetics Age estimation DNA methylation Methylation -sensitive restriction enzyme Subtractive hybridization
CLC: D919.2
Type: Master's thesis
Year: 2011
Downloads: 25
Quote: 0
Read: Download Dissertation

Abstract

Background individuals age estimation has been the focus and difficulty of the study of forensic science. More current practical application is the detection of bones, teeth and other bone indications, the application of forensic anthropology model calculations to infer the age of the individual. This method is more mature, and the error is also smaller. But encountered only blood or soft tissue samples case, due to the lack of indications for bone, its application would be extremely limited. With the development of molecular biology, in order to achieve through the detection blood or soft tissue to infer individual age, some scholars have proposed to infer individual age and telomere length and mitochondrial DNA damage. Both methods have some reference, is still difficult to be applied to the practice of forensic science, but because of limited accuracy. In recent years, with the rise of epigenetics research, the researchers found that DNA methylation in genomic abundant, widely distributed, and the close correlation between age. Therefore, if we can filter out the fragment of DNA methylation changes with age, and build a mathematical model between the ages, there will be possible to infer the age of the individual through the detection of DNA methylation status, is expected to infer individual age provides a new technical means. The purpose of individual genomic DNA methylation status by comparing different ages, fragment screening age changes in methylation, to further explore the correlation between DNA methylation and age lay the foundation. Two the methods select \Not Ⅰ digested genomic DNA of the two groups connect fittings restriction fragment amplified by PCR fragment amplified products using methylation-sensitive restriction enzyme. First amplification product was digested fragments of the old age group as Tester, youth group restriction fragment amplification products as Driver forward subtractive hybridization. After two to three subtractive hybridization (SSH), you can get the elderly relative juvenile differentially methylated DNA fragments. And then to the youth group restriction fragment amplified products as Tester elderly group restriction fragment amplification products as Driver reverse subtractive hybridization, juvenile relative aging differentially methylated DNA fragments. Finally, two sets of genes screened fragment purification, cloning, sequencing, identification of candidate target fragments and by methylation sequencing to verify the extent of the difference. (1) the use of methylation-sensitive restriction enzyme Not Ⅰ by reverse subtractive hybridization, initial screening methylation differences exist between the elderly and adolescents 10 the candidate target DNA fragment; (2) methylation sequencing shows that the screening out of the 10 candidate target fragment between 3 in different elderly and Juvenile group having a different degree of methylation differences. Conclusion \This article is the final screening out three with aging differentially methylated fragments laid a solid foundation for the further use of DNA methylation differences inferred individual age.

Related Dissertations

CLC: > Political, legal > Legal > UNIVERSITY > Forensic > Forensic Laboratory Science
© 2012 www.DissertationTopic.Net Mobile