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Background and objective experimental Diabetes mellitus is a systemic disease in which vascular lesions is its major complication. Previous studies have shown that non-enzymatic glycation in diabetes prevalence organization, excessive non-enzymatic glycation and advanced glycation end products (advanced glycation end products, AGEs) accumulation on the body structure, especially the large vascular endothelium, has extensive damage. In recent years, with the AGEs lead to vascular lesions have been carrying out research on the understanding of vascular injury caused by AGEs also increasing depth. This study was designed to observe and explore the diet and streptozotocin (streptozocin, STZ)-induced diabetic rats joint body, advanced glycation end products (AGEs) on vascular structure and function and aminoguanidine ( Aminoguanidine, AG), valsartan (Valsartan, VT) protection. In this study include the following: the establishment of diabetic rats and to detect whether the model is successfully established, observed in the different experimental groups AGEs rat aortic endothelial deposition situations, observing AGEs, aminoguanidine, valsartan on rat aortic diastolic functional effects were observed AGEs, aminoguanidine, valsartan on rat aortic structure. METHODS diabetic rat model, and given aminoguanidine, valsartan as an intervention factor, using immunohistochemistry and ELISA assay in rat aortic endothelial AGEs deposition conditions, with arterial rings in vitro rat primary research arterial vascular reactivity changes, the use of electron microscopy in rat aortic tissue ultrastructural changes were observed using Western blot method of eNOS protein expression in rat aorta for quantitative detection. Results: 1. Immunohistochemistry and ELISA results showed: diabetic rat aorta AGE content increased, and more present in aortic endothelial tissue; diabetic rats were given aminoguanidine intervention can reduce the formation of AGEs aorta; valsartan diabetic rat aortic intervention can not affect levels of AGEs. 2 Experimental results show artery rings: Diabetic rats NA (noradrenalin, norepinephrine) vasoconstrictor responses were significantly enhanced, while Ach (Acetylcholine, ACh) was significantly decreased diastolic blood; given aminoguanidine, Valerian sand Tan intervention can reduce the diabetic rats such a change, that reduce the sensitivity of the NA, increase sensitivity to ACh. 3 aortic ultrastructure: Diabetic rat aortic endothelial tissue ultrastructure pathologic changes, and aminoguanidine, valsartan tissue in diabetic rat aortic endothelial ultrastructure improvement over the former. 4.Western blot analysis showed: diabetic rat aortic tissue eNOS protein expression was decreased; aminoguanidine, valsartan can increase eNOS protein expression in aortic tissue. Conclusion diabetic rats increased levels of AGEs aorta, damage the structure and function of the aorta, aminoguanidine can reduce the deposition of AGEs, valsartan did not reduce the deposition of AGEs, but both drugs on the structure of the aorta in diabetic rats and function play a protective role.
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