Dissertation > Excellent graduate degree dissertation topics show

Expression of Netrin-1 mRNA in the Retina of Diabetic Rats

Author: ZhangXia
Tutor: XuHuiZhuo
School: Central South University
Course: Ophthalmology
Keywords: netrin-1 diabetic retinopathy diabetic rats
CLC: R774.1
Type: Master's thesis
Year: 2011
Downloads: 17
Quote: 0
Read: Download Dissertation

Abstract


Objective To detect the expression of Netrin-1mRNA in retina of diabetic rats and to evaluate the relationship between Netrin-1mRNA and diabetic retinopathy.Methods 20 adult Sprague-Dawley (SD) rats were randomly divided into two groups, Diabetic model group (DM) and Normal control group (NC),10 rats respectively. Streptozotocin (STZ) in freshly sodium citrate buffer (PH 4.5) was injected into peritoneal cavity in a dose of 60 mg/kg to induce diabetes mellitus. Rats in the control group were injected with the same volumes of sodium Oitrate buffer (approximate 0.5mL). Blood glucose levels higher than 16.67mmol/L were considered to be diabetic rats. Blood glucose and weight were measured every month. The technique of reverse transcription polymerase chain reaction (RT-PCR) was used to detect the expression of Netrin-1mRNA in retina respectively after STZ injection.Results (1) The success ratio of diabetic rat models was 90%, for one was failed to induce diabetes mellitus. The remained 9 diabetic rats appeared classic symptoms of diabetes mellitus, such as polydipsia, polyphagia, and so on, except the blood glucose. There was significant difference of blood glucose level between DM group and NC group (p< 0.01). There was significant difference of weights increase between two groups. Cataract was seen in the DM group at 3 month after STZ injection. (2) The result of RT-PCR revealed little expression of Netrin-1mRNA in the retina of control group rats. But a considerable increasing expression was seen in DM group (p<0.05).Conclusion Diabetic rat models can be successfully established by a single intraperitoneal injection of streptozotocin (60mg/kg). The expression of Netrin-1 mRNA in the retina of Diabetic rat models are significantly higher than that of the control group.

Related Dissertations

  1. The Effects of Atorvastatin on Expression of Osteopontin in Kidney of the Diabetic Rats Its Molecular Mechanism,R587.2
  2. Study on Therapy and Mechanisms of the Role of Tang Kang in Diabetic Rat and Endothelial Cells,R285.5
  3. Diabetic retinopathy OK vitrectomy surgery intraocular lens implantation in different periods of clinical observation,R779.66
  4. Effects of Columbin on Hepatic Drug Metabolizing Enzymes CYP450 in Experimental Type 2 Diabetic Rats,R965
  5. The Expression of ER-associated Degradation Related Factors in the Retina of Diabetic Rats,R774.1
  6. Qi Ming Treatment of Moderate to Severe Non-proliferative Diabetic Retinopathy Study,R276.7
  7. Theoretical and Clinical Study on Treatment of Diabetic Retinopathy Based on the Collateral Theory,R259
  8. Structure Change of Macular Arch in Diabetic Retinopathy,R774.1
  9. The Study of Mechanisms of Myopia on Preventing Diabetic Retinophty,R587.2
  10. Clinical Observation on Diabetic Retinopathy Treated by Acupuncture Combined with Oral Administration of TCM,R276.7
  11. The Study of Clinical Applications for Supra Scan Laser Photocoagulation in Diabetic Retinopathy,R774.1
  12. To Observe the Clinnical Effect of Da Ming Yin Unitedcalcium Dobesilate Treating Diabetic Retinopathy,R774.1
  13. Analysis the Relationship of Branch Retinal Vein Occusion、High Myopia and Asymmetric Diabetic Retinopathy,R774.1
  14. The Effect and Significance of Deguelin on the Expression of Hypoxia Inducible Factor-1α(HIF-1α) in the Retina of Diabetic Rats,R285
  15. Laser with traditional Chinese medicine treatment of diabetic retinopathy (DR) in clinical studies,R774.1
  16. Expression Changes of VEGF、nNOS in the Retina of Diabetic Rats Induced by STZ,R774.1
  17. Type 2 Diabetes Mellitus with Metabolic Syndrome and Diabetic Microangpathy,R587.2
  18. GABA-R with the Glu-R in diabetic retinopathy in the mechanism of action,R774.1
  19. Change and Significance of Markers to Prethrombotic State in Patients with Type 2 Diabetes Retinopathy,R774.1
  20. Diabetic nephropathy and diabetic retinopathy,R259

CLC: > Medicine, health > Ophthalmology > Retina and optic nerve diseases > Retinal disease
© 2012 www.DissertationTopic.Net  Mobile