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Objective clinical common gram-negative bacteria and antimicrobial resistance distribution, and the composition and distribution of multi-resistant case. Meanwhile against clinical drug resistance in Gram-negative bacteria β-lactamase gene TEM, SHV and CTX-M and disinfectant-resistant gene qacE △ 1-sul1 presence status to be analyzed. Method (a) with disc diffusion Kirby-bauer (KB) Determination of Air Force General Hospital from 2008 to 2009 in inpatient isolated from Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and Acinetobacter Total 1374 Acinetobacter antimicrobial drug resistance, and its resistance is analyzed. (2) using polymerase chain reaction (PCR) assay 155 common clinical multidrug-resistant bacteria: ESBLs-producing Escherichia coli and Klebsiella pneumoniae, multidrug-resistant Pseudomonas aeruginosa and Bowman Acinetobacter TEM, SHV, and CTX-M three kinds of β-lactamase gene and disinfectant resistant gene qacE △ 1-sul1. Results (1) within two years of the CCP specimens from hospitalized patients with a variety of purposes pathogens isolated from 1374. 384 E. coli, Klebsiella pneumoniae 263, both ampicillin resistance rates more consistent, both in 90% to imipenem and meropenem and other carbapenems and containing inhibitors antimicrobial susceptibility; against other antibiotics significantly higher resistance of Escherichia coli Klebsiella pneumoniae. Pseudomonas aeruginosa 530, more sensitive enzyme inhibitor containing antibiotics and amikacin, ceftazidime and cefepime, resistance rates of 30% or less; cefoperazone, levofloxacin and imipenem strong resistance, resistance rates of 40% or more. Acinetobacter baumannii 197, more sensitive to antimicrobial drugs carbapenems and amikacin resistance rates were below 30%, carbapenems are less than 20%, for the 3rd and 4th generation Cephalosporin antibiotics are less sensitive. Separation of ESBLs producing Escherichia coli, Klebsiella pneumoniae producing ESBLs, multi-drug resistant Pseudomonas aeruginosa and multidrug-resistant Acinetobacter baumannii total of 230, total detection rate of 16.7%. Mainly in neurosurgery, intensive care, endocrinology and other eight departments. (2) 50 ESBLs-producing E. coli, 41 TEM gene-positive (82.0%), seven SHV gene-positive (14.0%), 16 CTX-M gene positive (32.0%). 21 more than two genotypes both positive bacteria. 27 ESBLs-producing Klebsiella pneumoniae, 21 TEM gene-positive (77.8%), 24 SHV gene-positive (88.9%), three CTX-M gene positive (11.1%). 16 more than two genotypes both positive bacteria. In the 50 multi-drug resistant Pseudomonas aeruginosa 37 TEM gene-positive (74.0%) were not detected SHV and CTX-M genes. 28 multi-drug resistant Acinetobacter baumannii TEM gene positive in 25 (89.3%), 2 SHV-positive (7.1%), were not detected in CTX-M genes. Disinfectant-resistant gene qacE △ 1-sul1 in the four strains of bacteria were detected, 37,24,46,28, the detection rates were 74.0%, 88.9%, 92.0 and 100%, the overall detection rate of 87.1 %. Conclusion Escherichia coli, Klebsiella pneumoniae, and Acinetobacter baumannii to carbapenems and inhibitor-containing compound is sensitive to antibiotics. Pseudomonas aeruginosa enzyme inhibitor compound containing antimicrobial susceptibility, and strong resistance to imipenem. The multi-drug resistant isolates mainly in old age, interventional therapy or in patients with severe underlying diseases department. In the four kinds of multi-drug resistant Gram-negative bacteria β-lactamase gene detection rate is higher, indicating that as the broad-spectrum antimicrobial drugs widely used in clinical practice, the phenomenon of multi-drug resistant bacteria growing. Should pay attention to the distribution of pathogens and regular monitoring of drug resistance to guide clinical rational use of antimicrobial drugs to effectively control the infection and reduce the emergence of resistant bacteria. Disinfectant-resistant gene qacE △ 1-sul1 high detection rate, how scientific and effective use of disinfectants put forward new requirements.
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