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PBDEs (Polybrominated Diphenyl Ethers, PBDEs) is a brominated flame retardants , their wider application has caused global environmental pollution, penta-and eight - brominated diphenyl ethers have been included in the POPs Convention . To certain environmental issues is a hot topic in the current environment science , analytical methods and pollution research on PBDEs in China is only in its infancy . In this paper , the basis of the laboratory conditions for gas chromatography / electron impact ionization mass spectrometry and gas chromatography / negative chemical ionization mass spectrometry two detection methods were compared. Highlights the superiority of the negative chemical ionization , established of the GC-NCI/MS detection method . At the same time the conditions of polybrominated diphenyl ether samples before processing carried contrast optimization , using microwave extraction , silica / alumina composite chromatography column of sediment samples and biological samples carried purification , eliminating the other target multi- polybrominated diphenyl ether there is interference with the substance , to achieve a accurate quantitation . And the establishment of the system of quality control and quality assurance assessment to verify the feasibility and accuracy of the method . The application analysis established method of the seven PBDEs monomer of five different reaches of the Yangtze River Estuary near-shore and the Huangpu River sediment sediment in the determination of lower brominated diphenyl ethers in sediment samples content of 50.5 to 1236.3pg / g ( dw , dry weight) , less pollution of the Yangtze River estuary and nearshore Huangpu River PBDEs , mainly to lower brominated diphenyl ethers . In this paper, toxic , wider distribution of BDE-47 monomer , represented by polybrominated diphenyl ethers carp toxicity studies . Through acute toxicity test , come to BDE-47 semi- lethal concentration of 9.5 mg / L , chronic experiments , different concentrations of BDE-47 , BDE-47 exposure time carp liver CAT activity . The results show that the first received induction of CAT activity increased after received reduced inhibition . Also studied for 15 days , BDE -47 concentration ( 0.5 mg / L , 5 mg / L ) , fish BDE-47 enrichment trend detected in fish concentration of BDE-47 , pre-treatment methods and testing methodology similar methods and sediments .
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