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The Study on the Synthetic Origin and Receptor Distribution of PGE_ 2 and Its Related mRNA Expression During the Parturient Period of Goat

Author: GuGuoSheng
Tutor: GeLiJiang
School: Shandong Agricultural University
Course: Clinical Veterinary Medicine
Keywords: Goat Cervix PGE2 IHC real-time RT-PCR
CLC: S827
Type: Master's thesis
Year: 2011
Downloads: 17
Quote: 0
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Abstract


Cervix is an important organ that maintains gravidity and carries out the delivery in the female animal, cervical ripening is not only an active biochemistry process, but also an inflammatory reaction as there are a lot of media have participate in cervical ripening, such as prostaglandin, progesterone, relaxin etc., Prostaglandin E2 (PGE2) exerts a wide variety of physiological actions in mammalian reproduction, PGE2 receptors are classified into four subtypes, EP1, EP2, EP3 and EP4. PGE2 synthetic source and receptor distribution and relative express quantity of PTGS2,PTGER2,PTGER4 mRNA was researched in this project with Western blot, IHC and real-time RT-PCR method.The mechanism of cervical ripening in mammals and PGE2 synthetic source in cervix remanins obscure, although it is known that PGE2 is a critical mediator invovled in the mechanism. It is partly because information anout the location of PGE2 production and its EP receptors distribution in cervix is not suffieient. To reveal the mechanism, EP2 and EP4 receptor and PGE2 production immunolocalization in caprine cervical tissues in oestrus cycle, during pregnancy and at parturition was assayed by IHC and indirect immunofluorescence, and it is demonstrated whether adipocytes exist in cervix at various stages with Oil Red O staining. In additions, this project also researches prostaglandin-endoperoxide synthase 2 (PTGS2) mRNA, prostaglandin E2 receptor EP2 subtype (PTGER2) mRNA and prostaglandin E2 receptor EP4 subtype (PTGER4) mRNA in goat cervix (Periods of nonpregnant, Middle of gestation period and Stages of labor) with SYBR Green I real-time RT-PCR. The results suggest that EP2 and EP4 receptors are widely distributed in blood vessels, epithelium of cervical canal,circular and longitinal muscle, and stroma in cervix at parturition. There are strong EP2 receptor expression in cilia of epithelium and little expression in stroma and glandular epithelium in cervix at estrus.The PGE2 production occurs in endothelium of blood vessels, epithelium of cervical canal, circular and longitinal muscle, stroma and adipocytes in cervix at parturition, but negative location of PGE2 production and EP2 and EP4 receptor in cervix at proestrus, postestrus, diestrus and gestation. The relative express quantity of PTGS2、PTGER2 and PTGER4 mRNA in cervix at parturition is highest than two other period (extremely significant difference, P<0.01), gestation is lowest among them. It is important that adipocytes appear in cervix at parturition and can produce PGE2. And this result identify with real-time RT-PCR. Moreover, the changes in inflammation also occurs in cervix at parturition including leukocytes infiltration, edema, vascular dilation, collegen fiber break etc. wIHCh can be responsible for muscular relaxation and dilation. The changes in the amount of PGE2 production and the number of PGE2-producing cells, and distribution of EP2 and EP4 receptors can be responsible for cervical dilation in goats.

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CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Livestock > Goat
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