|
This test Isolation and identification of anthocyanin stability Japanese red maple (Acer palmatum) as test materials, respectively, of the Japanese red maple leaf pigment composition, temperature and light interaction on the impact of the Japanese red maple leaves fluorescence parameters and antioxidant enzyme activities under high temperature and irradiation buffer solution and Fe2 solution of Japanese red maple leaf photosynthesis and leaf antioxidant enzyme activities. Japanese red maple leaf pigment composition Isolation and identification of anthocyanin stability experiments in the sunny morning of 9: 00 1 0: 00 collected twigs middle leaf color reaction analysis, according to the Japanese red maple leaf flavonoid Japanese red maple leaf pigment composition, chlorophyll under different temperature, light, pH, metal ions and other conditions, changes in the anthocyanin content analysis of its stability. Temperature and light interaction on the Japanese red maple leaves fluorescence parameters and antioxidant enzyme activities affect the experiment is growing consistent, robust growth of Japanese red maple branches into Sheng nutritious liquid barrel, after the high temperature and high light (35 ° C, the PFD The 1200μmol · m -2 · s -1 ) low temperature glare (10 ℃, PFD 1200μmol · m -2 · s -1 ), low-temperature low-light (10 ° C, the PFD 100μmol · m -2 · s -1 ), high-temperature low-light (35 ° C, the PFD 100μmol · m -2 · s -1 ) treatment, n = 3 repetitions, Japanese red maple leaves fluorescence parameters and antioxidant enzyme activity in the determination process. High temperature and bright light buffer solution and Fe2 solution is to choose the Japanese red maple leaf photosynthesis and protection of enzyme activity in vivo experiments robust plants, respectively T1 - control (sprayed with distilled water), T2 - foliar spray pH5. 4 buffer solution, T3 - the foliar spray Fe2 (10 mmol · L -1 ) solution, T4 - foliar spray pH5.4 buffer solution of Fe2 (10 mmol · L -1 ) processing solution for the four groups, spraying every evening, the solution evenly sprayed on the leaves being the back to form a visible droplet for the degree. Two days after treatment began drawn drawn once a day, after six consecutive times. Each repeated 3 times, Japanese red maple leaf photosynthesis and leaf antioxidant enzyme activities of four treatments. The main findings are as follows:, Japanese Red Maple Leaves pigment containing anthocyanins, carotenoids, flavonol-O-3-glucoside, may contain flavonols, tea chalcone; stronger the light, the higher the temperature, anthocyanin the glycosides degradation faster; conducive to the stability of the anthocyanin the pH4.5 about most, to acidic or partial alkali will accelerate the degradation of anthocyanins; variety of metal ions can slow down the degradation of anthocyanins, Fe 2 The most obvious effect. 2, high temperature and high light, high temperature, low light for 5 d after the Japanese red maple leaf chlorophyll content reduced by 29.1%, 22.9%; high temperature and high light anthocyanin content decreased by 6%; temperature and intensive treatment 90 min after the blade Fo Fm, Fv / Fm decreased to handle 80.5%, 11%, 36.9%; high temperature and strong light, high temperature and low light leaf lutein loop library reduced by 9.7%, 7.3%, (AZ) / (VAZ) ratio increased by 66.2%, 61.9%; most antioxidant high temperatures cause the Japanese red maple leaf photoinhibition induced Japanese red maple leaf xanthophyll cycle started; within the high temperature and high light, low temperature to high temperature and low light will affect the Japanese red maple leaves activity of three processing the normal growth of the Japanese red maple leaves by a certain degree of stress. 3 separate spraying buffer solution and at the same time spraying buffer solution the the Fe 2 solution can make the SOD, POD, CAT activity increased (alone except spraying buffer solution of SOD) to ease suppression of the high temperature and high light three activity; separate spraying buffer solution the the Fe 2 solution and at the same time spraying both of the Japanese red maple leaf soluble sugar content increases, but the soluble protein content has little effect; spraying buffer solution alone will lead to an increase in proline content within the Japanese red maple leaves; addition to spraying distilled water alone, the other three sets of processing can not ease the Japanese red maple leaf chlorophyll degradation under high temperature and strong light conditions, increase net photosynthetic rate, but it can alleviate to some extent the degradation of anthocyanins spraying buffer solution of a set of the most obvious. Separately spraying the buffer solution to make PAL activity increased, the low pH of the intracellular fluid.
|