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Background: The rice germplasm in rice breeding research material basis for a correct and reasonable selection of excellent resources, is one of the key technologies to improve breeding effectiveness. The world's rice germplasm resources of about 10 million copies, and the end of 2003, China's total catalog of more than 70,000 copies of rice germplasm resources, but has been the backbone of the effective use of not more varieties, mainly concentrated in a handful of varieties which the derivative system. In the process of evolution of the system, with constantly adding new genes, the genetic background of the backbone varieties has also been continuously enriched, which provides a good selection of material for cross-breeding. A good parent material, in addition to the rich and excellent genetic characteristics, but also has the ability to pass this trait to their offspring. Breeding should understand its parental traits and trait gene composition, select their ecological types, genetic relationship exists some differences, geographic distance far the pro-this, it can make up for shortcomings of Backbone Parental, contributed to good character of mutual combination, to conducive to the restructuring of the gene, which achieved higher results. With the development of modern biotechnology, genomics technology, Rice Parents from traditional morphological and agronomic traits is the depth to the genome, transcriptome and proteomics direction. In this study, a survey of nine protein expression in different developmental stages Rice backbone parent protein expression polymorphism evidence for further parental choice and functional studies provide data resources. Research purpose: the backbone of the parents is an important foundation for breeding, Backbone Parental protein helps to understand its formation mechanism and promote its application, its function studies lay the foundation for different period of growth and development of the study of the expression characteristics of the different parent. Materials and Methods: select nine rice gene of of GST and BBTI8, with the expression of the full length protein antigen preparation, Myb been predicted fragment expression of the protein antigen prepared, the amount of expression of the protein, and purity are to meet the requirements of the antibody was prepared. Polyclonal antibody immune animal preparation of the protein using recombinant protein antigen test to verify the specificity of the antibody, the remaining six protein-specific antibody was pre-prepared in the laboratory [31]. This study by Western blotting techniques to investigate the six rice Backbone Parental (9311, Peiai, 64s of Teqing Zhenshan 97B, the Guanluai 4, and Dwarf Nantes) in different growth stages / parts (seedling aboveground found underground, photosynthesis, active oxygen scavenging and stress defense flowering stage flag leaf and tassel, mature flag leaves and ears) 9 protein expression of these proteins between different parents and different periods of site The expression polymorphism provide important reference data for the next functional studies. Results: GST, Myb and BBTI8 obtained after in vitro Cloning and Expression of the high purity of the protein, specific antibodies, and Western blotting revealed most of the protein expression levels in rice leaves high and relatively stable, little change in parental , while the expression patterns of change in OEE Ⅰ, GAPDH, SBPase, FBPA, GST, Myb and BBTI8 the seedling stage underground flowering stage ears and mature seeds showed higher polymorphism between different parents, including Article whether the belt, the strength of the increase or decrease the emergence of the new strip, the expression of these proteins Polymorphism Reaction of the characteristics of the different affinity, can be used as a species identification of a new tag, in addition, protein expression at different developmental stages / polymorphism and tissue-specific parts, reflecting the spatial and temporal specificity protein expression. On this basis, it is possible to gradually establish immunological detection system based on protein expression characteristics for species identification and screening of the field. Conclusion: This study prepared more important rice protein-specific antibody, the expression of these proteins in different parents polymorphisms were identified by Western blotting techniques, especially in the underground, spike, seeds and other parts of the expression quite different , expressed in leaves relatively close. In addition, the protein expression polymorphism, reflecting the spatial and temporal specificity of protein expression in different developmental stages / parts, apparently these antibodies can be used for functional studies of related proteins. In this study, a preliminary study ways to establish a use of protein-specific antibodies understand rice protein expression of specific protein polymorphism analysis. The prepared antibody-related functions, such as co-immunoprecipitation, Chip-on-chip pull-down and stress response accumulated resources, also can make use of the route system to understand the specific gene family specific metabolic pathways protein expression analysis.
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