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Objective: protein - energy malnutrition (protein-energy malnutrition, PEM) is due to lack of energy and (or) protein caused by a nutritional deficiency, mainly seen lt; 3-year-old infant. PEM clinical manifestations: weight gain so that mitigate the initial performance of malnutrition. Duration of persistence length (high) is also lower than normal. The subcutaneous fat layer is not fulfilling or completely disappear. The order of the abdomen, followed by chest - back - waist, and upper limb - lower extremities - hips, the final amount - neck - cheek. A large number of subcutaneous fat disappears, the skin becomes dry, pale, relaxation and wrinkles, loss of elasticity, the intestinal type can be seen. Muscle hypoplasia, hypotonia, or occasionally higher. Motor retardation, mental retardation, hypothermia, low heart sound blunt, rhythm arrhythmia, low blood pressure, breathing shallow. The forwards can affect the development of the intellectual and athletic qualities. Therefore, the children of the PEM should pay sufficient attention. So far, there is no specific laboratory parameters for early diagnosis of PEM. Proteomics (Proteomics), an emerging discipline developed in recent years by analysis to study the organization of the state of health and disease, cell populations and subcellular structures mitochondrial protein changes its intracellular presence of the protein and its activities for study and research cells express the similarities and differences of the protein under different physiological or pathological conditions, analysis of the relationship between the structure and function of proteins and protein, and thus to identify the function of proteins and specific proteins to further elucidate the pathogenesis of the disease and in-depth studies on the basis of effective therapeutic agents for the synthesis of target orientation. In this study, using protein chips combined with surface enhanced laser desorption ionization flying time mass spectrometry (surface enhanced laser desorption effect / ionization-time of flight-mass spectrometry., SELDI-TOF-MS) technology detects PEM treatment before and PEM treatment after the group expression has significantly with differences of protein, especially the low-molecular-weight proteins or peptides, hoping to find the reference value of serum protein in the early diagnosis of PEM, to provide a reference for the early diagnosis of PEM and PEM treatment, so that children can be treated as soon as possible. The purpose of this study is to explore the value of proteomics in PEM identify differentially expressed proteins, to provide alternative methods for the treatment of. : Application of SELDI-TOF-MS technology to analyze detect binding of serum proteins in the protein chip, a total of 68 specimens of serum low molecular weight protein expression fingerprint of PEM serum protein expression fingerprint of 56 cases, 12 cases of PEM After treatment, the serum protein fingerprinting. Results: 5 molecular mass / charge ratio (mass-to-charge ratio, mass electron ratio, M / Z) the 2000-20000 range of PEM and PEM between treatment groups was statistically significant (p <0.01) protein peak protein expression before and after treatment ((?) ± S) followed by 5314.4 (42.23 ± 4.09; 27.35 ± 4.51), 6417.8 (10.05 ± 2.76; 30.05 ± 4.23), 6616.9 (20.02 ± 3.42; 34.03 ± 6.23), 7742.8 ( 57.45 ± 9.72; 39.57 ± 4.15), 10216.5 (47.45 ± 9.63; 31.75 ± 2.73), M / Z 5314.4 (42.23 ± 4.09; 27.35 ± 4.51), 7742.8 (57.45 ± 9.72; 39.57 ± 4.15) 10216.5 (47.45 ± 9.63; 31.75 ± 2.73) in PEM group of high expression; M / Z 6417.8 (10.05 ± 2.76; 30.05 ± 4.23), 6616.9 (20.02 ± 3.42; 34.03 ± 6.23) higher in the PEM treatment group expression. Conclusion: 1, through on the test PEM protein metabolism; PEM treatment of children before and after the protein has changed, the PEM children may defect or imbalance due to the expression of proteins in the body caused by certain protein content than normal children treated complement proteins required for protein balance; 3, the use of chip joint mass spectrometry PEM protein metabolic characteristics may open new avenues for PEM molecular diagnostics, biological treatment, the pathological mechanisms.
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