Dissertation > Excellent graduate degree dissertation topics show

Study on the Association of the Pathological Damage and Clinical Indicators and Transformation Growth Factor Beta 1 and Smad7 Protein in IgA Nephropathy

Author: GaoLiFang
Tutor: WeiJianPing
School: Shanxi Medical
Course: Pathology and Pathophysiology
Keywords: IgA nephropathy Oxford typing Transforming growth factor-β1 smad7
CLC: R692.3
Type: Master's thesis
Year: 2011
Downloads: 71
Quote: 0
Read: Download Dissertation

Abstract

Purpose: IgA nephropathy (IgA nephropathy, IgAN) since 1968, Berger first reported, has been considered to be the world's most common glomerular disease. Lesion type complex with mesangial matrix increase, fibrotic lesions glomerulosclerosis (Sclerosis) stage of development to the final, so scientific, reasonable the renal histological type system helps compare IgAN renal tissue lesions and prediction of clinical outcome. The purpose of this project is to analyze the relationship between IgAN renal histopathology parting with renal clinical indicators; investigate serum IgA concentration, the relationship between the amount of IgA deposition in renal tissue and IgA nephropathy; observation of transforming growth factor β1 (TGF-β1) and Smad7 In the expression of the different types of IgA nephropathy, and its relationship with glomerular IgA immune complex deposition amount for the further study of the pathogenesis of IgA nephropathy and explore the TGF-β1 / Smads signal pathway in IgA nephropathy progress role. Method: 1, the collection of clinical data by clinical and renal biopsy confirmed 139 cases of primary IgA nephropathy patients. Clinical data including: age, gender, systolic blood pressure, diastolic blood pressure, 24-hour proteinuria, serum creatinine and serum IgA concentration. Calculate the estimated glomerular filtration rate (eGFR) and mean arterial pressure (MAP), renal biopsy specimens of 139 patients under the light microscope ratings, ratings standard reference IgA nephropathy Oxford typing. And compare the clinical indicators difference between pathological parameters and common sub-type group. 3, double sets by immunofluorescence staining with confocal laser microscopy detection of IgA immune complexes in different types of IgA nephropathy glomerular deposition. Comparison in the differences between the various pathological parameters and analyze the correlation among the indexes. 4, immunohistochemistry two-step detection of TGF-β1 and smad7 protein in tissues of IgA nephropathy wear, and compare these two proteins in the expression of the different types of IgA nephropathy and IgA immune complexes in the glomerular The deposition of the relationship between the amount. Results: IgA nephropathy tissue lesions with clinical indicators of the relationship between. The univariate analysis showed: In addition to the different groups of endothelial cell proliferation but (E0 / 1), the remaining parameters between the groups were statistically significant (P gt; 0.05) differences in clinical indicators and different degrees of correlation; 9 groups common sub-type group difference was statistically significant clinical indicators. 2, serum IgA concentration, the amount of IgA deposition in renal tissue and IgA nephropathy Oxford pathological parameter group between serum IgA concentration difference was not statistically significant; only mesangial cell proliferation assessment (M0/M1) between the two groups of IgA immune complex deposition the difference was statistically significant and positively related to the amount of immune complex deposition and renal tissue IgA; no correlation between the amount of serum IgA concentration and renal tissue IgA immune complex deposition; 9 groups were compared, no significant difference in the concentration of serum IgA, kidney tissue IgA immune complex deposition amount of the difference was statistically significant. 3 different types of IgA nephropathy TGF-β1 and Samd7 protein expression (immunohistochemistry) results of TGF-β1 and protein Samd7 brownish yellow granules positive expression in the cytoplasm of mesangial cells and tubular epithelial cells ( Figure 3-1). 9 group comparison between TGF-β1 and samd7 proteins in renal tissue difference is statistically significant (P lt; 0.05). 4, TGF-β1, Smad7 and pathological parameters and IgA immune complex correlation between the expression of TGF-β1 protein and glomerular segmental sclerosis the / Balloon adhesions (S), tubular atrophy / interstitial fibrosis ( T) was positive related (r = 0.349, P lt; 0.001; r = 0.325, P = 0.001); the expression of Smad7 protein's expression and mesangial cell hyperplasia score (M) was positive related (r = 0.213, P = 0.013) expression of TGF-β1 protein in renal tissue expression of Smad7 expression positively correlated (r = 0.279, P = 0.001), with IgA immune complex deposition amount of positive correlation (r = 0.322, P = 0.014), and Smad7 protein expression in renal tissue no correlation (r = 0.269, P = 0.094). latter Conclusion: IgA nephropathy Oxford typing four pathological parameters can more objectively reflect the dynamic indicators of kidney disease. . 2, the level of serum IgA concentration may not be IgA nephropathy occurs decisive factor, but excessive accumulation kidney tissue deposition of IgA immune complexes may glomerular mesangial extracellular matrix, play an important role in the process of glomerulosclerosis . IgA nephropathy glomerular deposition of IgA immune complexes may improve glomerular TGF-β1 protein expression of TGF-β1 and its signal transduction molecules Smad 7 is likely to participate in the lesions of glomerular extracellular matrix excessive accumulation, and play an important role in the process of glomerular sclerosis.

Related Dissertations

CLC: > Medicine, health > Surgery > Urology ( urinary and reproductive system diseases) > Kidney disease > Nephritis
© 2012 www.DissertationTopic.Net Mobile