Dissertation > Excellent graduate degree dissertation topics show

Regulation of Adiponectin mRNA Expression by Glargine, Detemir and Human Insulin in 3T3 - L1 Adipocytes

Author: LiangYu
Tutor: YangJing
School: Shanxi Medical
Course: Endocrinology
Keywords: Insulin glargine Detemir Human insulin Adiponectin 3T3-L1 adipocytes cells
CLC: R587.1
Type: Master's thesis
Year: 2011
Downloads: 45
Quote: 0
Read: Download Dissertation

Abstract


Objective: To investigate glargine , detemir and human insulin, adiponectin mRNA expression in 3T3-L1 adipocytes cells . Methods: 1. Cultured in vitro 3T3-L1 preadipocytes , induce differentiation and maturation of the basal medium after cultured for 24 hours , in order to eliminate the effect of the differentiation medium ; added followed containing 1nmol / L , 10nmol / L , 100nmol / L , 1000nmol / L human insulin ( pure) , insulin glargine ( pure and injection ) , detemir ( injection ) basal medium cultured 24 h, and the establishment of a basic medium group was the control group , cultured 24h , using real-time quantitative reverse transcription - polymerase chain reaction (RT-PCR) assay fat cells adiponectin mRNA levels . 2 . Statistical analysis: Data are expressed as mean ± standard deviation (x ± s) ; quantitative data compared using one-way ANOVA , pairwise comparisons with LSD-t test or Dunnet's method . P ≤ 0.05 , a statistically significant difference . Results: 1. Effects of different concentrations of the same insulin on adiponectin mRNA expression : different concentrations ( of 1, 10, 100 and 1000 ) nmol / L insulin action in 3T3-L1 adipocytes cells mature 24h, with insulin concentrations increase in adiponectin mRNA expression decreased gradually . Insulin concentrations ≤ 10nmol / L , the reduction of adiponectin expression was not statistically significant ( P gt; 0.05 ) ; concentration ≥ 100nmol / L , the expression of adiponectin decreased significantly , with statistical significance ( P lt ; 0.05) and 1000nmol / L insulin inhibition stronger than 100nmol / L insulin . The same concentration of different insulin Comparison of the effects of adiponectin mRNA expression : 10nmol / L groups relatively undifferentiated . 100nmol / L, 1000nmol / L the inhibition of insulin detemir ( injection ) inhibition weaker than insulin glargine ( pure and injection ) and human insulin ( pure) ; glargine insulin ( pure and injection ) weaker than human insulin ( pure ) ; Gan feedstocks undifferentiated products compared with glargine injection group . Conclusion: a high concentration (100 nmol / L , 1000nmol / L ) glargine , detemir and human insulin to both inhibit adiponectin mRNA expression in 3T3-L1 adipocytes cells in a dose - dependent manner . Detemir inhibition weaker than insulin glargine and human insulin ; inhibition of insulin glargine weaker than human insulin .

Related Dissertations

  1. A Basal and Clinical Reseach Ofω-3 Polyunsaturated Fatty Acids to Seal Non-alcoholic Fatty Liver Disease,R575.5
  2. Clinical Therapeutic Effect and Safety of Insulin Determir in Type 2 Diabetes Patients,R587.1
  3. Effects of A-SAA on the Expressions of Inflammatory Cytokines mRNA , Protein and Related Kinases of 3T3-L1 Adipocytes,R587.1
  4. Elderly heart failure patients with serum adiponectin , BNP, TNF-α correlation and its clinical significance,R541.6
  5. The Effect of Serum Adiponectin and Inflammatory Reaction on Malnutrition in Peritoneal Dialysis Patients,R692.5
  6. Coronary plant stand Hourui Shu atorvastatin experimental restenosis prevention clinical study,R541.4
  7. Studies on the Changes of Adiponectin, Resistin and Adipocyte Fatty Acid-binding Protein in Abdominal Obesity and Their Clinical Significance,R589.2
  8. Adiponectin Inhibits KISS-1 Gene Transcription and Expression Through AMPK and Sp-1 in the Hypothalamic GT1-7 Neurons,R587.1
  9. Changes of the Adiponectin and Resistin Levels in Burn Serum and the Role of Anti-inflammatory Drugs,R644
  10. Clinical Significance of Plasma Adiponectin Level in the Patients with Gastric Cancer,R735.2
  11. Experimental Study on Anti-inflammatory Effects of Aerobic Exercise on the Obesity,R87
  12. Spironolactone Influences the Expression of Adiponectin and PPARγ in Adipose Tissue of Type 2 Diabetic Rats.,R587.1
  13. The Study on Some Biochemical Indices of Young Women Wrestlers under Different Exercise Load in Weight Control and Loss Period,G804.4
  14. Metabolic syndrome TCM syndromes and pathological basis of,R259
  15. Adiponectin and Hypertension Study on relationship between TCM,R259
  16. High-fat diet -induced obesity and obesity-resistant rats expression levels of adiponectin Comparative Study,R589.2
  17. The Changes and Influential Factors of Adiponectin、Apelin and Visfatin in Coronary Heart Disease Patients Accompanied with Abnormal Glucose Metabolism,R587.1
  18. Study of Correlation between Serum Adiponectin Level and Arteriosteogenesis in Patients with Osteoporosis,R580
  19. The Analysis of Serum Adiponectin and TGF-β1 in Newly Diagnosed Type 2 Diabetic Patients with Microalbuminuria,R587.1
  20. Effects of Insulin on Adiponectin and AdipoRs mRNA Expression in Cultured Bovine Adipocytes,Q813
  21. SNPs Detection of Adipoq and Adipor and Its Relationship with Carcass and Meat Quality Traits in Qinchuan Cattle,S823

CLC: > Medicine, health > Internal Medicine > Endocrine diseases and metabolic diseases > Islet disease > Diabetes
© 2012 www.DissertationTopic.Net  Mobile