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Objective To study the susceptibility testing room Pseudomonas aeruginosa 30. Resistance, its resistance to β-lactam antimicrobial genotype to provide a reference for clinical experience in the use of antimicrobial drugs. Specimens and methods: 1. Susceptibility testing room extract from February 2010 to August 2010 hospitalized patients, postoperative incision secretions drainage fluid, blood, pus, ascites, isolated specimens 30 Pseudomonas aeruginosa, susceptibility testing using a universal disk diffusion method (kB Act), to detect antimicrobial resistance of 30 Pseudomonas aeruginosa strains frequently used for clinical performance. 2 real-time quantitative PCR (Polymerase Chain Reaction) reaction to detect resistance genes. Results: 1.30 Pseudomonas aeruginosa, the number 003,007,014,016,017,018,019,021,022,023,024,025,026,027,028,029, a total of 16 bacteria on more than 5 (five kinds) of antimicrobial resistance, the performance for the pan-resistance, accounting for 53.3% of all strains. Antimicrobial resistance rates were: amikacin (AMK) 53.3%, gentamicin (GEN) 40% the cefepime oxime (FEP) 23.3% 36.7%, Cefoperazone (CFP) cefoperazone / sulbactam (CSL) 20%, ceftazidime (CAZ) 40%, ceftriaxone sodium (CRO) 33.3% the aztreonam Southern (ATM) 40%, piperacillin / tazobactam (TZP) 33.3% , ciprofloxacin (CIP) 40%, 23.3% of imipenem (IPM), United States meropenem (MEM), 20% L-ofloxacin (LEV) 36.7%, polymyxin E (CL) 36.7%. The 2.β-lactamase gene detected in 16 (53.3%) manifested as pan-resistant Pseudomonas aeruginosa strains were not detected blaNDM-1, blaOXA-24, of blaSHV detected four positive strains (25%) blaDHA genetic testing positive strains 11 (68.75%), blaOXA-23 gene testing positive strains in 2 (12.5%), 7 blaIMP-1 gene detection Yang-resistant strains (43.75%). 3 cell outer membrane channel protein the transcriptional gene oprD2 gene detected 16 performance for pan-resistant Pseudomonas aeruginosa strains oprD2 missing 7 (43.75%). Observation drug susceptibility test results show: 7 Pseudomonas aeruginosa strains showed resistance to imipenem (imipenem). Conclusions: 1. Pseudomonas aeruginosa produce β-lactam enzyme is one of the main mechanisms of β-lactam antimicrobial agents. 2 clinically in the case of the face of severe infections of Pseudomonas aeruginosa, can be empirically select the combination preparation of the third generation cephalosporins, and β-lactamase inhibitors.
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