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Objective: To investigate atrophy Erosive Oral Lichen Planus (oral lichen planus, OLP) patients with Candida albicans detection rate, and isolates of Candida albicans genotyping and susceptibility studies to gene level for the clinical treatment of atrophic Candida albicans infection associated with patients with erosive OLP provide the basis for reasonable selection of antifungal agents. Method: 1. Selected 185 cases of atrophic erosive OLP patients (male 78 cases, female 107 cases) and 120 healthy control subjects (male 54 cases, female 66 cases) for the study. Improved cotton swab sampling, the CHROMagar Candida chromogenic medium and polymerase chain reaction (polymerase chain reaction, PCR) technology Candida albicans were separated and identified. 2 using the PCR method, transfer intron primers were designed for a section of the 25S rDNA of C. albicans, Candida isolates were genotyped on the white in the two groups. According to the American Society for Clinical Laboratory Standards Association (Clinical and Laboratory Standards Institute, CLSI) microdilution method recommended reference scenario (M27-A3) Determination of Candida albicans different genotypes of four clinical commonly used antifungal susceptibility. Results: 1, two groups of subjects balanced comparable. Isolation and identification atrophic erosive OLP group Candida albicans detection rate of 54.6%, higher than the 30.8% of the healthy control group, the difference there was a significant (P lt; 0.05). CHROMagar Candida chromogenic medium and PCR identification of Candida albicans was 100%. Atrophy erosive OLP group Candida albicans were detected in A, B, C three genotypes, each genotype proportion were 38.6%, 16.8%, 44.6%, to A, C-type; healthy controls the group Candida albicans also detected in the A, B, C three genotypes, genotypes proportion of, respectively, 91.9%, 5.4%, 2.7%, accounting for the vast majority of the A type. Candida albicans genotype constitute two groups there was a significant difference (P LT; 0.05). The 3.B type does not appear 5 - fluorocytosine resistant strains, A-5 - fluorocytosine resistance rate was significantly higher than B and C type (P lt; 0.05); B-type fluconazole resistance rate significantly higher than that of type A (P 0.05). Conclusion: 1. Atrophy erosive OLP group of white Candida detection rate was significantly higher than the healthy control group, compared with healthy people, atrophy in patients with erosive OLP oral microenvironment changes, making the Candida albicans easy adhesion colonization in atrophy the erosive OLP patients oral mucosa and blooms. Atrophy erosive OLP group Candida albicans genotype polymorphic, compared with the healthy control group, Candida albicans genotype changes. Atrophy in erosive OLP group and the healthy control group Candida albicans genotype constitutes the existence of significant differences, suggesting that atrophy in patients with erosive OLP associated with Candida albicans infection exogenous infection. 3. Atrophy erosive OLP white Candida different genotypes of antifungal resistance. Genotyping and susceptibility testing results, treatment should be reasonable selection of antifungal agents, in order to shorten the course of treatment and improve efficacy. All genotypes atrophy the erosive OLP white Candida nystatin almost no resistance, its clinical application should receive enough attention.
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