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Objective: Ulcerative colitis (ulcerative colitis, UC) is an infectious non-specific inflammatory disease, belongs to inflammatory bowel disease (inflammatory bowel disease, IBD) one, its etiology and pathogenesis is not yet clear. Study confirmed proinflammatory and anti-inflammatory cytokine imbalance plays an important role in the occurrence and development of IBD, cytokines can activate NF-κB, and the promoter region of a variety of pro-inflammatory cytokines have NF-κB sites, it is cytokines release the key regulatory factors. Blocking NF-κB signaling pathway, reducing the production of cytokines, inflammatory mediators, reduce inflammation, treatment of IBD new ideas. The experiment will curcumin for treatment dextran sulfate sodium (dextran sulphate sodium, DSS)-induced ulcerative colitis mouse model, detection of NF-κB activation and inhibition of protein IκB expression and cytokine TNF-α, IL -6 concentration observed efficacy of curcumin treatment colitis model, to explore its possible anti-inflammatory mechanism ulcerative colitis. Methods: 60 healthy female BALB / c mice, the mice aged 6-7 wk, body weight 18-22 g, were randomly divided into four groups, each group of 15. Group A: normal control group, the daily intake of drinking water and fodder, not treated for a total of 7 days; Group B: DSS colitis group, daily free drinking 5% DSS solution, daily intraperitoneal injection of group C volume in 25 ml / L ethanol solution, a total of 7 days; C Group: curcumin treatment group, drinking 5% DSS solution, once daily intraperitoneal injection of curcumin suspension (each 30mg/kg dose was dissolved in 25 ml of / L ethanol 1000μL), 7 d; D groups: dexamethasone treatment group, drinking 5% DSS solution, daily intraperitoneal injection of dexamethasone injection (each dose of 0.4mg/kg dissolved in saline 1000μL) , 7 d. All mice were sacrificed after 7 d specimens from colonic tissue to send pathology, another stay stored in liquid nitrogen for Western blotting and ELISA. Daily records of the mice in each group disease activity index (DAI) score, HE staining mouse colon mucosa histological changes. Measured by ELISA colon tissue tumor necrosis factor (TNF)-α, interleukin-Su (IL) -6 content. Immunohistochemical staining of nuclear factor-κB (NF-κB) expression in colon tissue inflammatory cells. Western blotting analysis colonic histoplasmosis NF-κB inhibition protein IκB expression. Results: 1 DAI score: group A: 0.37 ± 0.52, group B: 7.67 ± 1.56, group C: 1.64 ± 0.92, group D: 2.80 ± 0.92, ratings shown in group B and group C, D group difference was statistically significance (P lt; 0.01). Group C and Group D also statistically significant (P lt; 0.01). 2. Histological score: B Group the mice colonic inflammation involving the mucosa and submucosa, a few up serosal layer, incomplete colonic mucosa, visible and more multifocal shallow ulcers, the majority of the gland is destroyed, loss of normal gland structure seen intensive infiltration of inflammatory cells. Group C, group D mouse colon glandular destruction lighter structure is basically back to normal, inflammatory cell infiltration depth shallower than that in group B were also significantly reduced the number of inflammatory cells. Score in group B (2.83 ± 0.83) was significantly higher than that in group C, group D score (1.36 ± 0.50,2.00 ± 0.67), the difference was statistically significant (P lt; 0.01); C group score lower than Group D difference was statistically significant (P lt; 0.01) 3. colonic mucosa of TNF-α, IL-6 concentration was measured: the B group, TNF-α, IL-6 concentration [(102.75 ± 3.52) pg / ml ( IL-6 concentration was statistically significant (P lt; 0.01). NF-κB expression in mouse colon tissue: A group in the cytoplasm with occasional expression, B group the cytoplasm and nucleus showed a strong expression of group C in the cytoplasm, little expression in the nucleus, D groups in the cytoplasm and nucleus were expressed, but weaker than the B group. Group B score (7.92 ± 1.24) and group C (2.73 ± 0.79), D group (4.22 ± 1.09) difference was statistically significant (P lt; 0.01), C group and D group, the difference was statistically significant (P lt; 0.05). 5. colon tissue IκBα protein expression: Western blotting IκBα protein levels in colonic tissue of group B compared with group A decreased protein levels of the C group, D group and B group was significantly higher than the difference statistically significant (P lt; 0.05). CONCLUSION: Curcumin can effectively treat DSS-induced ulcerative colitis in mice, its efficacy is superior to dexamethasone. The mechanism may be through the inhibition of NF-κB signaling pathway and regulation of the cytokine TNF-α, IL-6, etc. release to play the role of curcumin is expected to develop a new drug.
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