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The Erythrocyte Damage and Its Mechanism in Rats Exposed Low Levels Arsenic

Author: ZhangJing
Tutor: PeiQiuLing
School: Shanxi Medical
Course: Health Toxicology
Keywords: Low concentrations Arsenic poisoning Rats Erythrocyte Erythrocyte membrane protein
CLC: R114
Type: Master's thesis
Year: 2011
Downloads: 58
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Abstract

Objective: 1. Analysis of relatively low concentrations of arsenic exposure on rat erythrocytes and erythrocyte membrane protein toxicity change, look for chronic arsenic poisoning early biomarkers; study of low concentrations of arsenic in rats exposed to damage the molecular mechanism of erythrocyte membrane protein for arsenic Study on the toxic mechanism to open up new ideas; 3 low concentrations of drinking water arsenic exposure in animal models, and determination of its toxic effects, and provide the basis for the safety evaluation of the national drinking water arsenic health standards. : 140g-160g healthy male SD rats 40, were randomly divided into four groups, namely the control group, 10μg / L, 60μg / L, 360μg / L arsenic exposure groups; 3 exposure groups with free drinking water containing exposed to arsenic in water, drinking ordinary clean tap water control group; exposed to 30 days, anesthetized animals, abdominal aortic blood samples were collected, anatomy whichever major organs. Analysis comparing different rats body weight, organ coefficient of general toxicity indicators. Detect different rats red blood cell parameters, erythrocyte Decline rate, red blood cell morphology and erythrocyte aggregation capacity and deformation capacity of red blood cell toxicity damage indicators. By SDS-PAGE electrophoresis and immunofluorescence technology analysis and comparison of the erythrocyte membrane protein changes. Results: 1. The rat general toxicity of different groups of rats at the same time weight difference was not statistically significant (P gt; 0.05); compared with the control group, 360μg / L arsenic treated rats spleen coefficients significantly increased, the difference was statistically significant (P lt; 0.05); no significant difference in the rest of the brain, heart, lung, liver, kidney coefficients between groups (P gt; 0.05). 2. The Erythrocyte toxicity 2.1 red blood cell parameters: Compared with the normal control group, the treated rats the erythrocyte parameters have not yet occurred abnormal change (P gt; 0.05). 2.2 erythrocyte Decline rate: flow cytometry results showed that, compared with the control group, 360μg / L arsenic treated rats increase in erythrocyte Decline rate (P lt; 0.05). 2.3 red blood cell morphology: arsenic exposure group rat red blood cell morphology abnormal changes of the cell surface appears irregular tiny protrusions or obvious spinous process changes, the most obvious changes 360μg / L arsenic exposure group, shows multiple irregular-shaped red blood cells as well as obvious spinous process shaped red blood cells. 2.4 erythrocyte hemorheological: Compared with the control group, 360μg / L arsenic exposure on whole blood low-cut apparent viscosity, relative viscosity and reduced viscosity were significantly lower (P lt; 0.05); 360μg / L arsenic exposure cut in the whole blood apparent viscosity decrease was statistically significant (P lt; 0.05); 360μg / L arsenic exposure group erythrocyte aggregation index significantly less than the control group (P lt; 0.01); different group high shear blood apparent viscosity, relative viscosity and reduced viscosity no significant change (P gt; 0.05), erythrocyte rigidity index and deformation index also no significant change (P gt; 0.05). Erythrocyte membrane protein damage by SDS-PAGE separation α-spectrin, beta-spectrin, ankyrin, band 3 protein band 4.2 protein, actin, a total of six major rat red blood cell protein. Compared with the control group, 360μg / L arsenic exposure rats anchor protein content decreased, and the difference was statistically significant (P lt; 0.01). Increased with the exposure dose, the rats in each group with 3 protein expression of an increasing trend, and 360μg / L of arsenic exposure groups were statistically significant (P lt; 0.05). The remaining four kinds of protein expression levels change, there was no significant difference (P gt; 0.05). Immunofluorescence test results show that the band 3 protein and CD35 in arsenic exposure rat red blood cell membrane rendering cluster increased more obvious to 60μg / L and 360μg / L arsenic exposure groups. Conclusion: The low concentrations of arsenic exposure of rat erythrocytes produce cytotoxicity, lead the increased erythrocyte Decline rate (eryptosis), red blood cell morphology abnormal changes reduced red blood cell aggregation, the reason may be arsenic-induced erythrocyte membrane band 3 protein The anchor protein content abnormalities and red blood cell membrane band 3 protein and CD35 clusters increased related. Low concentrations of arsenic exposure erythrocyte membrane protein damage compared with the cell number and volume changes appear earlier, erythrocyte membrane protein may be early biomarkers of chronic arsenic poisoning. And the spleen may be the early target organ toxic effects of low concentrations of arsenic exposure in rats.

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