Dissertation > Excellent graduate degree dissertation topics show

The Effect of Wnt3a Factor and Bone Morphogenetic Protein-2 Co-regulating Bone Marrow Mesenchymal Stem Cell Proliferation and Osteogenic Differentiation

Author: YeJin
Tutor: XiaoJun
School: Huazhong University of Science and Technology
Course: Surgery
Keywords: Wnt3a bone morphogenetic protein-2 marrow mesenchymal stem cells osteogenesis
CLC: R329
Type: Master's thesis
Year: 2011
Downloads: 16
Quote: 0
Read: Download Dissertation

Abstract


Objective: To observe the impact of classical Wnt/β-catenin and BMP signaling pathway effect on rat bone marrow-derived mesenchymal stem cell proliferation and osteogenic differentiation. Methods: Bone marrow mesenchymal stem cells were isolated by density gradient centrifugation combined adherence separation methods and divided into four groups: control group, Wnt group, BMP group, Wnt3a and BMP-2 combination group. Cell proliferation activity and alkaline phosphatase (ALP) activity were analyzed quantitatively by CCK8 protocol. Cellular osteogenic differentiation and matrix mineralization were decided by Von Kossa staining. The expression of osteoblast-specific markers were detected by RT-PCR. Results: At culture day 7, CKK-8 activity was 0.845 in combination group, 0.738 in Wnt3a group and significantly higher than 0.409 of control group (P <0.05). At culture day 6 and day12, cellular ALP activity was 63.8 and 144.3 of combination group, 40.8 and 104.1 of BMP-2 group, higher than 7.3 and 18.9 of control group (P <0.05). Cultured for 14 days, of combination group osteocalcin mRNA expression was significantly higher, while the expression of Runx2 and Osterix was not significant compared with other groups. Culture for 3 weeks, Von Kossa staining showed the number and size of calcium nodules of combination group were higher than of control group. Conclusion: Combined induction of Wnt3a factor and bone morphogenetic protein2 can effectively promote mesenchymal stem cell proliferation and osteogenic differentiation.

Related Dissertations

  1. The Construction and Evaluation of a Novel Non-viral Gene Transfection System and Its Application in Mesenchymal Stem Cells Gene Recombination,R346
  2. The Research on the Best Time of Bone Marrow Mesenchymal Stem Cell Transplant after Myocardial Infarction,R542.22
  3. Experiment on Repeated Bone Harvest Chamber in Vivo,R329
  4. The Effects of Trophic Effects of Bone Marrow Mesenchymal Stem Cells on Astrocytes Induced by Carbon Monoxide in Culture,R329
  5. BMP2 and VEGF165 Genes Transfected Rat Bone Marrow Mesechymal Stem Cells Simultaneity Expression,R329
  6. Application Adipose-devired Stem Cells and Silk Fibroin and Chitosan Scaffold Materials Building Common Cells-stents Complex Experiments,R329
  7. Experimental Investigation of Grafting Pathways of Bone Marrow Stromal Cells into Spinal Cord Injury in Rats.,R651.2
  8. Four-point bending stress stimulation of human bone marrow mesenchymal stem cell proliferation activity,R329
  9. Autologous bone marrow stem cell transplantation in the treatment of heart failure after myocardial infarction term efficacy,R542.22
  10. Bone marrow mesenchymal stem cells in rats with severe acute pancreatitis CD4 ~ CD25 ~ regulatory T cells influence and Integrative Medicine,R576
  11. Bone marrow mesenchymal stem cells in the same kind of heart transplantation on Treg cells in rats,R654.2
  12. Non-small cell lung cancer with BMP-2 expression and significance of CD105,R734.2
  13. Effect of H2 O2 Pretreatment of Autologous Mesenchymal Stem Cells Transplantation on Ventricular Remodeling After Acute Myocardial Infarction,R542.22
  14. Construction and Identification of PIRES-BMP2-TGFβ3 Bicistronic Eukayotic Expression Vector,R87
  15. The Expression and Significance of Wnt3A、Wnt5A and Wnt10B in Human Breast Carcinoma-associated Fibroblasts,R737.9
  16. Evaluation of Distraction Osteogenesis in the Treatment of Micrognathia Associated with Obstructive Sleep Apnea Hypopnea Syndrome,R782
  17. A Technique Innovation and Initial Animal Experimentation of the Distraction-implant,R782
  18. pEGFP/Ang-1 Transfected Rat BMSCs Transplantation on Diabetic Retinal Vascular Leakage of Retinal,R774.1
  19. pEGFP/Ang-1 Transfected BMSCs Attenuates High Glucose Concentration-induced Choroido-retinal VECs Damage,R774.1
  20. Induction of bone marrow mesenchymal stem cells on spinal cord injury in rats Experimental study of neurological recovery,R744

CLC: > Medicine, health > Basic Medical > Human morphology > Human histology
© 2012 www.DissertationTopic.Net  Mobile