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Effects of EGCG on the Voltage-gated Potassium Channels in Primary Cultured Hippocampal Cells

Author: ZhangWenCai
Tutor: ChenJuTao
School: University of Science and Technology of China
Course: Neurobiology
Keywords: EGCG Transient outward potassium currents Delayed rectifier potassium current Whole-cell patch clamp Hippocampal
CLC: Q42
Type: Master's thesis
Year: 2011
Downloads: 19
Quote: 1
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Abstract


Table epigallocatechin gallate (EGCG) is the main component of green tea. By widespread attention in recent years because of its antioxidant, anti-inflammatory, weight loss, anti-cancer and other effects. However, with the progress of the study found that the high concentration of EGCG in cultured hippocampal cells and in vitro brain film can cause some side effects, these the vice side effects may be related to the voltage-gated channels. In order to further investigate the regulatory role of the high concentration of EGCG on voltage-gated channel, we study the EGCG on primary cultured hippocampal neurons voltage gate to control the impact of the potassium channel, the main observation of EGCG on transient outward potassium current I A < / sub> and the regulation of delayed rectifier potassium current I K . Using whole-cell patch clamp technique, we studied EGCG transient outward potassium channel and delayed rectifier potassium channel current - voltage curve, activation curve and inactivation curves affect. Our data show that: (1) different doses of EGCG can significantly inhibit the I the A I K potassium current amplitude, and a concentration-dependent manner, and its median inhibitory concentration IC 50 , respectively from 251.70 ± 37.47μM and 181.8 ± 9.33μM. (2) EGCG I A , of the I K inhibition of voltage-dependent inhibition enhanced mobile with the voltage of the depolarizing direction. (3) EGCG so I A and I K state activation curve to hyperpolarized direction to move, making the channel easier to be activated in the more negative potential, the half activation voltage V1 / 2 moved 11.7mV and 6.4mV. (4) EGCG so I A and I inactivation curve K hyperpolarizing shift, half lost the live voltage V 1/2 The and moved 20.72mV and 14.86mV. Inactivation curves left that can be activated in a potential decrease in the number of channels, this may be a EGCG potassium current smaller. (5) will be activated and compare the extent of inactivation curve to the left, can be observed activation curve is greater than the inactivation curves of EGCG on I A and I K impact. EGCG potassium channel activation and inactivation voltage range is narrowed, so that the decrease in the number of channels can be activated, causing a decline in the current. (6) the effect of EGCG on I A and I K impact, whether the degree of mobile activation curve inactivation curve is the I A change is greater than I K of. This may EGCG, I the A I K channels in different ways caused. Nervous system, voltage-gated potassium current recovery has an important role in neuron excitability and action potential, potassium channel dysfunction causes neuronal firing rate changes in the characteristics and abnormal discharge. Our studies have shown that high concentrations of EGCG on primary cultured hippocampal neurons voltage gate control potassium channel I A and the activation and inactivation of I K obvious role . This suggests that we will EGCG used in weight loss, anti-cancer, anti-inflammatory when notice of EGCG for voltage-gated channels.

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