Dissertation > Excellent graduate degree dissertation topics show

Implantation of Mesenchymal Stem Cells (MSCs) Carried by LINK-N/RADA-16 to the Intervertebral Disc : A Study to Repair the Disc

Author: PengShuai
Tutor: ShaoZengWu
School: Huazhong University of Science and Technology
Course: Surgery
Keywords: Mesenchymal stem cells peptide self-assembly LINK-N/RADA-16 Degeneration Model
CLC: R687.3
Type: Master's thesis
Year: 2011
Downloads: 4
Quote: 0
Read: Download Dissertation

Abstract


Objective:①To investigate the effect of the Annular Puncture and Aspiration disc degeneration model;②To investigate the effect that LINK-N/RADA-16 carry MSCs to repair the degenerate intervertebral disc.Methods:Rabbit bMSCs were cultured in vitro and the Self-assembled peptide nanofiber material LINK-N/RADA-16 was compounded; the treatment to each intervertebral disc segment is in the following: L2/3-only Annular Puncture and Aspiration; L3/4-after puncture and aspiration RADA16 +MSCs was injected; L4/5-after puncture and aspiration LINK-N/RADA-16+MSCs was injected, L1/2 segment was as control.Before the treatment and three weeks later MRI was obtained, and then the rabbits were sacrificed for histological analysis and immunohistochemistry of the intervertebral disc tissue.Results:①MRI results showed that the height the L2/3 was lower and the image singal was decreased in the T2W, compared with that the degeneration of the L3/4 segment was mild while the L4/5 segment was near to the normal control group.②The histology and immunohistochemistry results showed that nucleus pulposus cells was in a small number in the L2/3 segment and fibrous cartilage degeneration occured, at the same time, the level of collagenⅡwas lower compared with the control group; in the L3/4 segment these changes were mild and the L4/5 segment was near to the control group.Conclusion:①The method of punture and aspiration can effectively cause animal disc degeneration;②Implantation of bMSCs carried by LINK-N/RADA-16 can effectively inhabit the disc degeneration, so this material can be used as a good scaffolds of the disc tissue engineering.

Related Dissertations

  1. Study of Intracerebral Transplantation of Umbilical Cord Blood Mesenchymal Stem Cells in a 6-Hydropamine Rat Model of Pakinson’s Disease,R742.5
  2. The Construction and Evaluation of a Novel Non-viral Gene Transfection System and Its Application in Mesenchymal Stem Cells Gene Recombination,R346
  3. The Protective Effect of Rat Myocardial Ischemia/reperfusion Injury Following Bone Marrow Mesenchymal Stem Cell Pretransplantation for 1 Week,R542.22
  4. The Study of Immuno-tolerance Mechanism of the Third-party Bone Marrow-derived Mesenchymal Stem Cells on Allogenic Transplantation,R392
  5. The Effect of Exogenous Brain-derived Neurotrophic Factor on Transplantation Treatment of Bone Mesenchymal Stem Cells in Intracerebral Hemorrhage in Adult Rats,R743.34
  6. The Research on Treatment of Lentivirus-mediated hVEGF-165 Gene Transferring Mesenchymal Stem Cells on Rat Hind Limb Ischemia,R329
  7. The Research on the Best Time of Bone Marrow Mesenchymal Stem Cell Transplant after Myocardial Infarction,R542.22
  8. Therapeutic Effect and Mechanisms of Derived Mesenchymal Stem Cells in Intracerebral Hemorrhage Rat,R743.34
  9. Effects of Ginsenoside Rg1 on Cell Proliferation, Secretion Function and ERK、p38 Signal Molecular in Human Umbilical Cord Blood Mesenchymal Stem Cells under Optimized Culture Condition,R285.5
  10. Comparison of Chondrogenic Potential between Mesenchymal Stem Cells from Rabbits’ Bone Marrow and Adipose Tissue,R329
  11. Human Adipose Tissue Derived Stem Cells Differentiate into Epidermal Cell and Fibroblasts,R329
  12. The Effects of Trophic Effects of Bone Marrow Mesenchymal Stem Cells on Astrocytes Induced by Carbon Monoxide in Culture,R329
  13. BMP2 and VEGF165 Genes Transfected Rat Bone Marrow Mesechymal Stem Cells Simultaneity Expression,R329
  14. An Experimental Study on the SDF-1/CXCR4 Expression of Infracted Myocardium in Rat after Transplantation of Hypoxia-preconditioned MSCs,R542.22
  15. Effects of Receptor Activity Modifying Protein-1 Transplantation of Mesenchymal Stem Cells on Postangioplasty Restenosis and Cardiac Function after Myocardial Infarction in Rabbits,R542.22
  16. Experimental Investigation of Grafting Pathways of Bone Marrow Stromal Cells into Spinal Cord Injury in Rats.,R651.2
  17. Bone Mesenchymal Stem Cells with Cytosine Deamimase Gene Transducted in Migrat to Glioma in Vivio,R739.4
  18. Four-point bending stress stimulation of human bone marrow mesenchymal stem cell proliferation activity,R329
  19. VEGF-induced neuronal differentiation into mesenchymal stem cells migration research,R329
  20. Autologous bone marrow stem cell transplantation in the treatment of heart failure after myocardial infarction term efficacy,R542.22
  21. Bone marrow mesenchymal stem cells in rats with severe acute pancreatitis CD4 ~ CD25 ~ regulatory T cells influence and Integrative Medicine,R576

CLC: > Medicine, health > Surgery > Orthopaedic Surgery ( movement system diseases,orthopedic surgery ) > Orthopedic surgery and surgery > Bone surgery
© 2012 www.DissertationTopic.Net  Mobile