Dissertation > Excellent graduate degree dissertation topics show

A Preliminary Investigation of the Expression of SK4 in Hepatocellular Carcinoma

Author: YangZuoZuo
Tutor: YiJiLin
School: Huazhong University of Science and Technology
Course: Surgery
Keywords: SK4 KCa3.1 hepatocellular carcinoma HCC TRAM‐34
CLC: R735.7
Type: Master's thesis
Year: 2011
Downloads: 2
Quote: 0
Read: Download Dissertation

Abstract


Objective: This research was to explore the expression and effect of SK4, an intermediate conductance calcium‐activated potassium channel, on hepatocellular carcinoma.Methods: Western Blotting was used to detect the expression level of SK4 protein in tissue samples. Real‐time PCR analysis was used to detect the transcription level of KCNN4 mRNA. The method of MTT was used to investigate the function of SK4 in human hepatocellular carcinoma cell in vitro. Specific blocker of SK4, tetraethyl‐ ammonium‐34, was used in this part to inhibit the proliferation of HepG2 cell. All data were analyzed by the statistics software of SPSS 16.0.Results: Comparing with para‐carcinoma tissue, an increase of the transcription and translation were detected in HCC tissue samples (P<0.05). The proliferation of HepG2 cell was inhibited by the specific blocker of SK4 in vitro. And the intensity of this effect is time and concentrate‐dependent.Conclusion: The transcription and translation were up‐regulated in the HCC tissue samples. And date from in vitro experiment shows that SK4 may have positive effect on the proliferation of human hepatocellular carcinoma cell. This effect can be inhibited by specific blockers. Thus SK4 may play a role in the progression of human hepatocellular carcinoma.

Related Dissertations

  1. Cloth and β- elemene combined administration of anti-tumor effect and mechanism of celecoxib,R96
  2. Clinical Significance of STAT3 and MMP-2 in Hepatocellular Carcinoma.,R735.7
  3. Assessment of Efficicy of Transarterial Chemoembolization by Gelfoam Particles Using DSCT Perfusion Imaging for Hepatocellular Carcinoma,R735.7
  4. Quantiative Proteomics of Liver Immortalized Cell Line Chang Liver and Liver Cancer Cell Line HepG2,R735.7
  5. Expression and Significance of NGF and Its Receptors in Primary Hepatocellular Carcinoma,R735.7
  6. The Impact of HBV on Oval Cells and Its Effects on the Expression of P8,R735.7
  7. Expression and Clinical Significance of SK4 in Hepatocellular Carcinoma, Breast Carcinoma and Thyroid Carcinoma,R730.4
  8. Influence of Synuclein-γ on the Effectiveness of Antimicrotubule Drugs in Human Hepatoma Cells and the Mechanism,R735.7
  9. TACE treatment of primary liver cancer long-term outcome and prognostic factors,R735.7
  10. Study on the Correlation between the Imaging Signs and Serological Index of HBV Related HCC.,R735.7
  11. The Single-phase Voltage-type PWM Rectifier Research Based on the Electric Locomotive Traction Application,TM461
  12. Anti-angiogenic therapy inhibits liver cancer growth and metastasis, recurrence and Mechanism,R735.7
  13. The Expression and Clinical Role of TIMP-2 in Hepatocellular Carcinoma in Hui Nationality,R735.7
  14. Diagnostic Value of Antibodies Against a Panel of Multiple Tumor-associated Antigens in Diagnosis of Primary Hepatocellular Carcinoma,R735.7
  15. PITX1 in hepatocellular carcinoma and its clinical significance,R735.7
  16. Expressions and Singnificate of Livin Gene and Its Relation with the Expressions of β-catenin in Hepatocellular Carcinoma,R735.7
  17. Primary Mechanism Study of Cell Growth Promoted by Microcystin LR,X173
  18. The Association of Survivin and COX-2 Gene with Hepatocellular Carcinoma,R735.7
  19. The Expression of SOX7 mRNA and Methylation of SOX7 DNA in Hepatocarcinoma Cell Lines and Hepatocarcinoma Tissues,R735.7
  20. PLAG1 and PLA2G2A abnormal expression in HCC,R735.7

CLC: > Medicine, health > Oncology > Gastrointestinal Cancer > Liver tumors
© 2012 www.DissertationTopic.Net  Mobile