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[ Objective] To establish Aeromonas Porphyromonas gingivalis (Porphyromonas gingivalis, Pg) infection in animal models , objective evaluation of Yunnan Baiyao Pg - induced bone destruction , and to provide new ideas for the prevention of inflammatory bone disease and periodontitis , treatment . [ ] Of the 50 -day-old healthy male 72 Kunming mice ( body weight 20 to 25 g ) were randomly divided into three groups : the model group (M = 24 ) , the Baiyao treatment group (Z = 24 ), and the control group ( group N = 24 ) . Model group , the Baiyao treatment group in the middle of the mice ears parietal to the occipital bone of bone inoculated with P. g1 × 108CFU/ml , 200ul twice injection , the control group was injected with the same dose of saline . The the Baiyao treatment group was given the day of the modeling Yunnanbaiyao gavage per 0.5 ml ( 12.5 mg ) , model group and the control group received the same dose of saline . Batches of each group were sacrificed 8 days 5,8,14 modeling using HE staining pathological examination of each group inflammation and bone destruction ; detected by ELISA in each group , serum TNF -α , IL - 6 content change ; TRAP staining detected skull osteoclast content ; RT-PCR was used to detect the the skull RANKLmRNA , expression of OPGmRNA , and the experimental results were statistically analyzed . [Results ] compare days 5,8,14 the Baiyao treatment group , model group and the control group of mice serum TNF - α , IL - 6 content , the number of osteoclasts and skull RANKL / GAPDH ratio 5th day of each index in the model group are the highest , and Baiyao treatment group lt ; model group difference ( p lt; 0.05 ) , compared to the two groups and the control group values ??are high differences ( p lt ; 0.05 ). OPG / GAPDH ratio comparison between groups found in the first 5,8,14 - day the Baiyao treatment group are significantly higher than the model group and control group difference ( p lt; 0.05 ) , and peaked in the first eight days ; the model group and the control group at day 5 model group than the control group difference ( p lt; 0.05 ) , day 8 model group was higher difference ( p lt; 0.05 ) , 14 days higher than different from the control group (p gt; 0.05). [ Conclusion ] Yunnanbaiyao inhibit bone destruction and bone repair significant role in to reduce blood IL - 6 , TNF - α expression ; to to downward adjustment expression of RANKLmRNA , raised OPGmRNA expression ; inhibition of osteoclast formation , thereby inhibiting the absorption of bone to promote bone repair .
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