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Effects of Adipose-derived Stem Cells Transplantation on Angiogenesis in the Brains of Rats with Focal Cerebral Ischemia and Its Mechanism Study

Author: LinFeiFei
Tutor: LiuZuo
School: Fujian Medical
Course: Neurology
Keywords: Adipose-derived stem cells Cerebral ischemia Angiogenesis Notch1-Dll4 signaling pathway
CLC: R743.3
Type: Master's thesis
Year: 2011
Downloads: 65
Quote: 0
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Abstract


Objective: To observe the adipose-derived stem cells (Adipose-derived Stem Cells, ADSCs) transplantation to improve the regeneration of blood vessels after cerebral ischemia ; detection of adipose-derived stem cells before and after transplantation , rat brain ischemic surrounding area Notch1-Dll4 signaling pathway expression change, to explore the signaling pathway in ADSCs transplantation to promote the role of the peripheral area of the cerebral ischemia revascularization , ADSCs provide experimental basis for the treatment of ischemic stroke . Methods: stickers wall filter were isolated , cultured , purified ADSCs before transplantation to the DAPI mark ; 65 adult male SD rats were randomly divided into : sham operation group (Sham group ) , model group ( MCAO group of ) the ADSCs intervention group ( ADSCs group ) , the ADSCs the DAPT intervention group ( the DAPT group ) ; reference modified Zea - Longa intraluminal filament middle cerebral artery occlusion (Middle Cerebral Artery Occlusion, MCAO) model ; the ADSCs intervention group and ADSCs the DAPT intervention group were made 24 hours after the model was successful in the stereotaxic instrument under injected into the left ventricle ADSCs or with gamma secretase inhibitor DAPT ( a specific inhibitor of the Notch signaling pathway ) ADSCs ; postoperative 4d , 7d , 14d, 28d decapitation take brain microvessels , counting each group cerebral ischemia surrounding area ; immunofluorescence method , Western blotting detection Notch1 , Dll4 , HES1 expression . Results : ADSCs after transplantation around the infarct observed positive cells scattered in the distribution of DAPI staining . Microvascular number of the ADSCs intervention group cerebral ischemia and the surrounding area was more than MCAO group ( P lt ; 0.05 ) and ADSCs DAPT intervention group ( P lt ; 0.05 ) ; the ADSCs intervention group were Notch1, Dll4, Hes1 protein expression levels compared with MCAO group and ADSCs the DAPT intervention group was significantly higher ( P lt ; 0.05 ) . CONCLUSION : ADSCs transplantation can significantly promote microvascular proliferation of rat brain ischemic surrounding area ; ADSCs transplantation can upregulate the expression of Notch1-Dll4 signaling pathway , this role can be inhibited by the Notch signaling pathway specific inhibitor DAPT ; ADSCs transplantation promote cerebral ischemia surrounding area angiogenesis may increase the expression of Notch1-Dll4 signaling pathway .

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CLC: > Medicine, health > Neurology and psychiatry > Neurology > Cerebrovascular disease > Acute cerebrovascular disease ( stroke)
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