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Matrix metalloproteinase-3 and its tissue inhibitor-1 expression in the rabbit lumbar bone defects and growth plate in

Author: XuQiMing
Tutor: XuLin
School: Beijing University of Traditional Chinese Medicine
Course: Traditional Chinese Medicine
Keywords: Bone growth plates Endochondral ossification Nano- hydroxyapatite / collagen composite Matrix metalloproteinase Tissue inhibitor of matrix metalloproteinase
CLC: R681.5
Type: Master's thesis
Year: 2011
Downloads: 29
Quote: 0
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Abstract


1 Background endochondral ossification is a bone tissue along the cartilage template growth process, which occurs in the process of bone development, fracture healing and bone growth plate of the bone formation process, and involves rich in type II collagen fibers and aggregation avascular cartilage proteoglycan rich zone to the type I collagen fibers and mineral-rich vascular bone tissue changes. Endochondral bone, cartilage cell precursors mesenchymal cells differentiate into cartilage cells, and subsequent proliferation of cartilage cells and become mature chondrocytes hypertrophy. Hypertrophy cartilage calcification, cartilage matrix degradation and vascular ingrowth hypertrophic chondrocytes began apoptosis, subsequent bone began to be deposited in the calcified cartilage trabeculae. Although a growing number of studies involving cartilage ossification matrix, but in which the various parts of the exact relationship is not yet clear. Bone healing is a complex process involving osteoid degradation and matrix mineralization, matrix metalloproteinases and their inhibitors plays an important role in this process. Regulation of the activity of matrix metalloproteinases by the tissue inhibitor of matrix metalloproteinases (TIMPs), TIMPs by non-covalent bond is formed bimolecular complexes and prevent the binding of MMPs precursor MMPs inactivation. TIMPs and MMPs play an important role in the occurrence of bone remodeling. Provides a good model of cartilage into bone to repair bone defects, because it simulates the growth step in the process of bone development. Bone defect repair is not only a process of cartilage and bone quickly generate, but also one of the extracellular matrix widely degradation process. MMP-3 and TIMP-1 expression in bone growth plates of bone defect repair process in the MMP-3 and TIMP-1 expression has reference value. MMP-3 and TIMP-1 expression in the the rabbit lumbar epiphysis in this study using immunohistochemistry observed changes over time, and to observe changes in the expression of MMP-3 and TIMP-1 in the process of bone defect repair, which distinction of osteogenic ability to judge the different bone material mediated bone repair. 2 Objective To observe the MMP-3 and TIMP-1 expression in the bone defect repair process changes, in order to determine the difference between osteogenic ability of different bone material mediated bone repair. 30 New Zealand white rabbits, 3 experimental methods, anterior transabdominal surgery revealed lower lumbar, the dental burr establish the rabbit lumbar 5/6, lumbar 6/7, waist 7 / S1 intervertebral defect model. L5 / 6 intervertebral defect implanted material, as a negative control group; the lumbar interbody 6/7 defect implantation of injectable nano-hydroxyapatite / collagen composite as the experimental group; lumbar / sacral intervertebral defect implantation has clinical use of solid nano hydroxyapatite / collagen composite, was used as a positive control group. Randomly selected animals, respectively, after 4 weeks, 8 weeks, 10 weeks, 12 weeks, 16 weeks, 20 weeks the animals were killed lumbar spine specimens produced by each animal L5 / 6, the lumbar 6/7, lumbar / sacral a tissue slice of the intervertebral area, with rabbit antibody MMP-3, TIMP-1 antibody, etc. an anti-processed, and then corresponding to the above antibody anti-antibody to be processed, and made a color processing, draw the tissue blocks specific area of ??the MMP-3 and TIMP-1 expression. 4 experimental results in the experimental group and positive control group, bone defects, mainly through endochondral bone repair. Chondrocyte expression of matrix metalloproteinase -3 (MMP-3) and tissue inhibitor of metalloproteinase -1 in the repair process. Over time, the expression levels of MMP-3 and TIMP-1 first increased and then the downward trend, the former peak in the 12 weeks after surgery, the latter peaks in 16 weeks after surgery. In the experimental group intervertebral bone defects filled with injectable nano-hydroxyapatite / collagen artificial bone material before the arrival of the peak expression of MMP-3 and TIMP-1 higher than the solid nano hydroxyapatite / collagen artificial bone material filling the positive control group the interbody bone defects in MMP-3 and TIMP-1 expression levels. 5 Conclusions injectable nano-hydroxyapatite / collagen artificial bone material mediated bone repair than solid nano hydroxyapatite / collagen artificial bone material mediated bone repair has a higher osteogenic ability.

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CLC: > Medicine, health > Surgery > Orthopaedic Surgery ( movement system diseases,orthopedic surgery ) > Bone diseases > Spine and back disorders
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