|
Objective: To explore the establishment of simple, economical and practical, stable and reliable rat animal model of benign bile duct stricture methods conducive to the occurrence of benign bile duct stricture mechanisms and effective treatment research. Methods: 49 female SD (Sprague Dawley) rats weighing 220-260 g, mean 240 ± 10 g, were randomly divided into experimental group 21, the experimental control group and blank control group, 21 7, all animals were with 3% of the intraperitoneal injection of pentobarbital anesthesia. Experimental group, 21 animals were anesthetized with intraperitoneal xiphoid epigastric abdominal incision to expose the common bile duct at a distance of 2cm at the upper edge of the duodenum isolated bile about 1cm, which is placed below a thickness of about 0.2cm After the infusion pad about 0.7ml of 95% ethanol for 2-3 minutes in the bile duct after the abdomen was closed; experimental control group in the same way for surgery, the bile duct on 0.9% saline infusion 2-3 minutes off abdomen; blank control group only to find out after bile duct abdomen was closed without additional processing. Were observed, including mental state, urine color, eating habits; experimental model group and the experimental control group after 14, 21 were taken three times a day is divided into seven animals weighed and timely blood tests of liver function (ALT , GGT, T-BIL, D-BIL), and cut part of the liver and bile duct experiments at pathological examination, 21 days cholangiography group also conducted to understand the situation of bile duct obstruction. T-test using SPSS statistical software, and multivariate analysis of variance. RESULTS: Postoperative 1hSD rats can stand independently activity ,3-4h after drinking, and gradually resume eating. ① from preoperative to postoperative 21 days, the experimental model group serum alanine aminotransferase (ALT), γ-glutamyl transferase (γ-glutamyl transferase, GGT), serum total bilirubin (T- BIL), serum direct bilirubin (D-BIL) and other indicators of significant change (P lt; 0.05); while the control group and blank control group did not change significantly. ② rats were sacrificed at each time point cut the liver and bile ducts pathological examination, laboratory-made modules over time found that there are varying degrees of liver cell damage, bile duct fibrosis tissue sections; while the control group and blank control group, preoperative and postoperative no significant changes in contrast. ③ cholangiography after 21 days found that experimental bile duct stricture made module performance; while the control group and blank control group without bile duct stricture appears. Through the above comparison, the experimental group and the control group was significantly (P lt; 0.05), control group and blank control group was not statistically significant (P gt; 0.05). Conclusion: the extrahepatic bile duct outside infusion of 95% ethanol manufacture of benign bile duct stricture rat model is feasible, can be benign bile duct stricture study provides another animal model.
|