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Background: paraquat (paraquat, PQ) also known as gram Wuhu track of paraquat, Bala Li, chemical name 1,1 '-dimethyl-4, 4'-dichloro-bipyridine, heterocyclic compounds belonging to the bipyridine belonging to the contact herbicide, is currently the most widely used herbicide in case of alkali decomposition, rapid loss of activity after contact with the soil. Paraquat has a very strong toxicity to humans and animals, the mortality rate as high as 50% -80% of acute poisoning, the reasons for the high mortality and pathogenesis is unclear and no effective detoxification drug-related clinical. Lung, liver, kidney, heart and are paraquat poisoning target organ damage. Performance in the early stage of acute kidney injury, serious, acute renal failure, and even death. The kidneys are vital organs of the human body, there are many physiological functions, the main excretory function, regulate water and electrolyte and acid-base balance, and can regulate blood pressure, can also renin secretion and promoting hormones such as erythropoietin. When paraquat poisoning, kidney as an excretory organ, the first damage, leading to a variety of dysfunction. Renal excretion of toxic substances in the body most of paraquat poisoning, poison and inflammatory substances from the kidney a large number of excretion. When renal dysfunction, although urine output may appear to be normal, but poison excretory function loss, of paraquat and inflammatory substances cause the body is unable to effectively discharge, which play a catalytic role in the liver, lung lesions, thereby affecting Paraquat poisoning prognosis. It can be seen that the treatment of kidney injury, PQ poisoning is a very important position, the integrity of the kidney function is to ensure that the basis of the effective treatment of other organs. Objective: clinical practice, the use of ulinastatin protect the kidneys effect, but less specific therapeutic effect of; Also, because there is no clear basis for clinical ulinastatin treatment dose selection, investigated the effects of Herbs blight (paraquat, PQ) poisoning pathological features of kidney damage, and by different dose ulinastatin treatment of PQ poisoning kidney injury, to explore the optimal dose of ulinastatin can initially achieve the following objectives: (1) clear paraquat poisoning different time kidney disease characteristics; (2) a clear therapeutic effect and dose-effect relationship of ulinastatin PQ poisoning kidney. Method: experimental selection of health experiments with Wistar rats 140, provided by the Experimental Animal Center of Jilin University, male or female, body weight (250 ± 20) g, the rats were randomly divided into seven groups, six groups of rats were prepared PQ poisoning model through the mouth, respectively, 2 times a day to give Group A: saline 1m1 twice daily intraperitoneal injection; Group: B exposure groups: saline 1m1 daily intraperitoneal injection; Group: C exposure groups: ulinastatin D 30,000 iu / kg twice daily intraperitoneal injection; Group: D exposure groups: ulinastatin D 60,000 iu / kg twice daily intraperitoneal injection; group E: exposure groups: Ulinastatin, 90,000 iu / kg twice daily intraperitoneal injection; F Group: exposure groups: ulinastatin 120,000 iu / kg twice daily intraperitoneal injection; G: exposure groups: ulinastatin 180,000 iu / kg daily twice by intraperitoneal injection, the rat the venous serum creatinine values ??taken on days 1, 3, 7, 14, to take kidney HE staining 1,3,7,14,21,28. Results: 1, kidney serious injury lesion tubular main acute phase and chronic phase gradually restored. Group B, the most serious lesions observed PQ poisoning the rat kidney HE staining found, manifested as glomerular bleeding, renal tubular cloudy swelling, lumen visible protein casts, renal tubular epithelial cells hyaline degeneration, interstitial inflammation infiltration, some tubular necrosis, structural disintegration visible renal interstitial hemorrhage. 2.CDEFG group of kidney disease was significantly lighter than control group exposed to creatinine values ??CDEFG group group were obviously lower than the exposure control group. 3, F, G group kidney lesions light C, D, E group, acute phase apparent, chronic phase difference smaller. Conclusion: paraquat poisoning, kidney serious injury lesion to renal tubular the specific pathology showed significant swelling of the renal tubular epithelial cells, glass stools seen renal interstitial inflammatory infiltration and hemorrhage protein casts and bleeding, while visible in the tubular chamber, glomerular injury is minor, visible the renal capsule a small amount of bleeding. The paraquat poisoning kidney injury is most evident in the acute phase, gradually reduce chronic phase. Ulinastatin of paraquat poisoning and kidney damage significant therapeutic effect, can effectively reduce the degeneration of tubular epithelial cells, and can reduce infiltration of renal interstitial inflammation and bleeding, while inhibition of creatinine increase . Larger dose ulinastatin treatment effect of paraquat poisoning kidney in the acute phase than small doses as well, statistically significant decreases more than 7 days after the significant difference between groups.
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