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Objective: To investigate the macrophage migration inhibitory factor (macrophage migration inhibitory factor, MIF) neutrophils (polymorphonuclear neutrophils, PMN) in rats with severe acute pancreatitis (severe acute pancreatitis, SAP) and Ulinastatin (the ulinastatin UTI) its role in the intervention. Methods: 52 healthy Wister rats were randomly divided into three groups: (1) normal control group (C, 12): open only flip the pancreas and duodenum, the abdomen was closed. (2) SAP model group (group A, 20): the duodenal papilla retrograde injection of 3.5% sodium taurocholate (sodium taurocholate STC)-induced rat model of SAP. 5 min (3) of UTI treatment group (U group, 20): duodenal retrograde injection of 3.5% sodium taurocholate preparation of rats with severe acute inflammation model, the portal vein to give Ulinastatin 40,000 U / kg . Each group postoperative 3H, 6h, 12h, 24h different points in the inferior vena cava after blood sacrificed, the cut pancreatic tissue. Measured by ELISA serum MIF levels detected by flow cytometry neutrophil apoptosis rate, under the light microscope parallel pancreatic pathological score line to observe the pathological changes of the pancreas, the routine determination of serum amylase levels. Results: (1) A serum MIF levels 3h has risen to a high value, in 6h, 12h, 24h is still at a higher level, a significant difference (p lt; 0.01) compared with group C and group U rats were given Ulinastatin, serum MIF levels compared with group A was significantly lower (p lt; 0.01); (2) compared with group C, A group of rats at each time point amylase levels and pancreatic tissue pathology The score was significantly higher (p lt; 0.01), and increased gradually over time, U serum amylase levels and pancreatic histology pathology score compared with group A, there was a significant difference (p lt; 0.01 or p LT; 0.05). (3) A group of neutrophil apoptosis rate than in group C was significantly lower (p lt; 0.01), and over time, A apoptosis rate gradually decreased to a minimum value of 59.40 ± 1.58, 24h, and the group U neutrophil apoptosis rate was increased (p lt; 0.01 or p LT; 0.05) than group A. (4) the correlation analysis, A serum MIF levels and pancreatic tissue pathological score was positively correlated (r = 0.513, p lt; 0.05), the level of neutrophil apoptosis in pancreatic histopathological scores were negatively correlated (r = -0.929, p lt; 0.01), serum MIF levels and neutrophil apoptosis rate was negatively correlated (r = -0.445, p lt; 0.05) Conclusion: (1) SAP, serum MIF levels significantly increased, rated as highly positive correlation, indicating that MIF may play an important role in the pathogenesis of SAP and pancreatic tissue pathology. (2) SAP neutrophil apoptosis rate with time decreased pancreatic tissue pathology score was negatively correlated, also participated in the pathophysiological process in SAP. (3) A serum MIF levels and neutrophil apoptosis rate was negatively correlated, suggesting that MIF may be aggravated by influencing the rate of neutrophil apoptosis in pancreatic tissue damage. (4) A group of rats were given Ulinastatin, serum MIF levels, the levels of amylase and pancreatic tissue pathological scores were lower, said Ming Wu cilastatin through inhibition of trypsin activity, down MIF levels, and promote neutrophil increased apoptosis rate, reduce the pathological damage of the pancreas, is an effective drug for the treatment of SAP.
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