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The Role of GOLPH2 in Liver Inflammation and Liver Damage

Author: LongZuo
Tutor: PengTao
School: University of Science and Technology of China
Course: Biochemistry and Molecular Biology
Keywords: GOLPH2 Hepatitis and liver damage IL - 1b IL-6 GOLPH2 knockout mice
CLC: R363
Type: Master's thesis
Year: 2011
Downloads: 49
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Abstract

Golgi phosphoprotein 2 (GOLPH2, GP73) is a function of the unknown Golgi type II transmembrane protein located in the cis-Golgi, and can be transported through the cell membrane system secreted into the extracellular matrix. Clinical and pathological studies have shown that the close relationship of GOLPH2 disease, especially hepatitis and liver cancer and hepatitis patients found have GOLPH2 abnormally high expression and secreted into the blood, which may be used as serological markers for clinical diagnosis of liver cancer. The the abnormal expression GOLPH2 mainly in clinical studies, and less cellular and molecular mechanisms for GOLPH2 abnormal expression of hepatitis and liver damage, which limits the GOLPH2 clinical and value. This study applied animal and cell models to study the process and mechanism GOLPH2 abnormal expression of hepatitis and liver injury, and to explore the significance of abnormal expression GOLPH2. Application hepatitis animal models and liver injury in animal models, you can track the occurrence and progression of hepatitis and liver damage. Study found that very rapid and sensitive the GOLPH2 response to hepatitis and liver damage, liver by stimulation of LPS or CCl4 24h, GOLPH2 of expression levels increased rapidly, and 48 hours after injury, reached a peak; hepatitis and liver damage repair coming to an end level of GOLPH2 to slow decline decline in GOLPH2 level may also be related with the prognosis. GOLPH2 abnormal expression of liver inflammation may be the regulation of inflammatory factors (cytokines), the use of cytokines in vitro stimulation of mouse primary hepatocytes and hepatoma cell line Hep3B cells detection GOLPH2 expression. The results showed that IL-1b and IL-6 has raised the GOLPH2 the role of TGF-b significantly down-regulated the liver expression of cell GOLPH2 in level; does not detect the moderating effect of TNF-a and IFN-r GOLPH2. This part of the study results showed that the process with hepatitis immune cells secrete IL-1b, IL-6 expression levels may GOLPH2 Relevance. To further study the expression of GOLPH2 hepatitis physiological significance of the existence of the first time we build and use GOLPH2 knockout mouse model, using the Cre-loxP system to knock addition to GOLPH2 gene exon 5 results display GOLPH2 there is a not yet found new alternative splicing, exon 6 5 'end there are two splice sites, making GOLPH2 can select the connection of two different locations of exon 6, belong to the alternative splicing of the variable 3' end of splice way. Due to the existence of alternative splicing is not known in advance, making GOLPH2 knock In addition to the mouse into the exon 5 deletion mutant mice, the study of exon 5 deletion mutation of individual reproductive developmental effects, and may reduce the liver stress ability. Based on the findings, liver GOLPH2 exception expression may be a stress in the form of the liver and in close contact with the damage repair process may be involved in this process, at the same time GOLPH2 there is a strong response mutant ability, we found GOLPH2 new Variable splicing Mode.

The Role of GOLPH2 in Liver Inflammation and Liver Damage

Abstract

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