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Diabetic nephropathy is one of the major microvascular complications of diabetes, the pathological structures mesangial hyperplasia, basement membrane thickening, characterized, and eventually glomerulosclerosis and interstitial fibrosis. P38 is one of the members of the MAPK family, played an important role in the development of diabetic nephropathy, it is involved in extracellular matrix fibrosis and many other physiological and pathological processes. Tongluo recipe (Tong, luo Recipe, TLR) is based on Chinese medicine collateral disease theory developed from the compound preparations by of ginseng, leeches, scorpion, centipede, Eupolyphaga and RPR drug, and preliminary studies show that our group has a protective effect on diabetic nephropathy, can improve endothelial cell function by inhibiting the RAS system, anti-oxidation, and other mechanisms play a role in the kidney of diabetic rats. Tongluo recipe, however, the protein diabetic kidneys fiber connection (FN) affect currently no reports. The experimental use of streptozotocin (STZ)-induced diabetic rat model, and to observe the impact of the TLR proteins and their pathologic structures of the kidney of diabetic rats P38MAPK-FN pathway, to further explore the possible mechanisms of TLR protective effect on diabetic kidney. This study through an automated biochemical analyzer to detect the normal control group, diabetic group, the Tongluo prescription intervention group rats blood sugar, blood urea nitrogen, serum creatinine, left kidney weight / body weight, 24-hour urine protein urine protein / creatinine ratio; renal cortex PAS staining; electron microscopy glomerular basement membrane; Western blot analysis detected t-p38MAPK, p-p38MAPK, t-CREB (cAMP response element binding protein, CAMP response element binding protein), P-CREB, FN (fiber connexin) protein expression, diabetes, renal dysfunction significantly increases glomerular mesangial proliferative, p-of p38MAPK, P-CREB, FN protein expression was significantly increased Tongluo recipe intervention significantly improved kidney down p-p38MAPK, P-CREB, FN protein expression. Tongluo recipe may have a protective effect in diabetic nephropathy and renal function by inhibiting p38MAPK-FN signaling pathway, Objective: To study TLR of STZ-induced diabetic rat kidney the p38MAPK-FN signal transduction pathway to explore TLR protective effect on diabetic kidney possible mechanisms. Methods: The subjects: health, specific pathogen-free (SPF) SD rats. CON control group (N = 7), diabetic untreated group DM (N = 7) and diabetes Tongluo recipe group DM TLR (N = 7). The first part: using automatic biochemical analyzer rat venous fasting blood glucose, blood urea nitrogen, serum creatinine, urine creatinine, RIA assay in 24-hour urine protein. Renal cortex PAS staining. Electron microscopy the glomerular basement membrane. Part II: Western blot analysis to detect renal cortical tissue t-of p38MAPK, p-p38MAPK, t-CREB (cAMP response element binding protein, CAMP response element binding protein), P-CREB FN protein expression. Results: 1. General: the rats were injected with STZ 1 week after tail vein blood to measure blood sugar gt; 16.7mmol / L, and determines the diabetic model were successfully modeling success rate of 86.7%. Thereafter once weekly monitoring of blood glucose, blood glucose remained l6.7 mmol / L or more, a good model of stability. 36 ~ 48 h after STZ injection in rats polydipsia, polyuria, 3 to 4 days, eat more significantly, lack of energy, dry hair, gloss down \CON rats eating, drinking water, urine normal, Mao Faguang bright, natural increase in body mass. Tongluo recipe after treatment of diabetic rats reaction is sensitive hair gloss increased \Biochemical parameters in rats compared with CON DM rats average left kidney weight / body weight ratio, blood glucose, blood urea nitrogen, creatinine, 24-hour urine protein, urine protein / creatinine increased significantly (p lt; 0.05). Compared with DM group, DM TLR group in addition to the blood glucose, blood urea nitrogen, these parameters are significantly lower (p lt; 0.05). DM group and DM TLR group of PAS staining of renal cortex, glomerular increased mesangial matrix, you can see the CON group did not show. Compared with DM DM the TLR rats matrix, mesangial proliferative less. Electron microscopy glomerular basement membrane DM group and DM TLR group, you can see the glomerular basement membrane thickening CON group did not show. Compared with DM DM the TLR rats glomerular basement membrane thickening less. 5 compared to the renal cortex t-p38, p-p38, t-CREB, P-CREB FN level CON group, DM group, t-p38, t-CREB no change (p GT; 0.05), p-p38 2.37 (p lt; 0.05), p-CREB increased 2.21 (p lt; 0.05), FN increased 2.03 (p lt; 0.05); compared with DM, DM TLR group was significantly lower p-p38, p-CREB, FN (p lt; 0.05); t-p38, t-CREB not changed (P GT; 0.05). Conclusion: Tongluo recipe may be by inhibition of p38-FN pathway in diabetic nephropathy have a protective effect.
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