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White and plastic rats on cervicitis EGF, EGFR and TNF-α expression

Author: ZhongHaiYing
Tutor: ZhangZuo
School: Hunan University of Traditional Chinese Medicine
Course: Chinese Gynecology
Keywords: Cervicitis White and plastic Epidermal Growth Factor Epidermal Growth Factor Receptor Tumor necrosis factor -α
CLC: R285.5
Type: Master's thesis
Year: 2011
Downloads: 73
Quote: 0
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Abstract


Objective: In this study, rats were white and glue on cervicitis intervention study of epidermal growth factor (EGF), epidermal growth factor receptor (EGFR) and tumor necrosis factor- α (TNF-α) expression in the role of cervicitis and white and plastic treatment of cervicitis mechanism . For the clinical treatment of cervicitis provide a theoretical basis . Methods: 25% of phenol glue vaginal administration , copying into a rat model of successful cervicitis , 60 rats were randomly divided into six groups , the rats were injected with saline vaginal blank matrix formulation , double-yl Thailand bolt , white and plastic low, medium and high dose ( equivalent to the human clinical dose equivalent dose of a 1x, 2x , 4x ) , day 1 , a total of 12d, the day after the end of treatment, the animals were killed , cut vagina to the uterus of the corner of organization , observation of cervical pathological changes in the overall organization , using immunohistochemical analysis assay EGF, EGFR and TNF-α in cervical tissues . Results: Compared with model group , white, and glue each group , double-yl Thai suppository group ( positive control group ) on the cervical tissue expression of EGF and EGFR levels , TNF-α levels decreased, were statistically significant (P lt; 0.05 ) , compared with the rest of the treatment group , white and plastic high-dose group had significant difference . Each treatment group compared with the corresponding parts of the model group , lesions and inflammation is reduced significantly , and no hemorrhage and necrotic tissue , epithelial hyperplasia basic repair , or even close to normal (P lt; 0.01); while the treatment group compared to the morphology of the cervix no significant difference in the impact study (P gt; 0.05). Compared with before treatment , significant increase in body weight of rats in each group were statistically significant (P lt; 0.05). Conclusions: These results show that 25% of phenol glue administered four times per 3d animal model successfully copied cervicitis cervicitis rat with white and gum treatment, symptoms improved significantly , the lesion tissue machine repair , or even close normal , that understand and plastic rats on cervicitis have a better therapeutic effect. White and plastic cervical tissue can promote the expression of EGF and EGFR , cervicitis tissue reducing the content of TNF-α , increased cervical tissue blood flow, and promote cervical squamous epithelial cell proliferation, differentiation , accelerate tissue healing , in which white and plastic high dose group effect is particularly significant. So that tends to balance damage and repair factors , which may be white, and glue one of the mechanisms for Cervicitis

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