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Soothing itching milk keratinocyte cell proliferation and the intervention effect on the the p38MAPK signal channel and VEGF levels

Author: HuangWenJuan
Tutor: LiuLiFang
School: Hunan University of Traditional Chinese Medicine
Course: Surgery of Chinese Medicine
Keywords: Soothing itching milk Keratinocytes Cell Proliferation P38 mitogen-activated protein kinase signaling channel Vascular endothelial growth factor
CLC: R285
Type: Master's thesis
Year: 2011
Downloads: 24
Quote: 0
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Abstract


Objective: To observe the soothing itching milk formed the proliferation of cultured human epidermal keratinocytes, explore soothing itching milk keratinocyte cell P38 mitogen activated protein kinase (p38MAPK) signaling pathway regulation of vascular endothelial growth factor (VEGF) expression of impact and p38 MAPK signal pathway and VEGF expression correlation between the two. Method: 1. Improved serum-free culture, cultured in vitro normal human epidermal keratinocytes forming cells, cell morphology was observed under an inverted microscope, and keratinocytes immunocytochemistry. 2-methyl thiazolyl tetrazolium the trace enzyme reaction assay (MTT method) measured soothing itching milk keratin formation on cultured cells without the side effects of cytotoxic concentration range. 3 times diluted in a non-toxic concentration range moisturizing milk itching, take four concentrations, additional tazarotene group inhibitor group and the control group, the MTT assay of cell proliferation. Western blotting (Western blot) determination of the phosphorylation of p38 content; enzyme-linked immunosorbent assay (ELISA) of VEGF secretion changes. Results: 1 cells cultured in vitro with horny cell morphology is formed by keratin positive staining. 2 soothing itching milk in 0-0.4855g/ml within the concentration range of keratin forming cells toxic side effects; compared with the control group, skin itching milk 0.4g/ml, 0.2g/ml and 0.1 g / ml concentration of keratinocytes significantly inhibited proliferation (P lt; 0.01) 0.05g/ml concentrations promote cell proliferation, but the difference was not statistically significant (P GT; 0.05) Tazarotene group, the inhibitory effect on cell proliferation in 0.4g/ml concentration difference was not significant (P gt; 0.05) 3 compared with the negative control group, the drug concentration in the 0.4g/ml 0.2g/ml, phosphorylation of p38 protein expression was significantly lower (P lt; 0.01); When the concentration 0.1g/ml, protein expression decreased (P lt; 0.05). Tazarotene group protein inhibitor group were significantly lower (P lt; 0.01) compared with the negative control group. (4) Compared with the control group, the secretion of VEGF When the drug concentration 0.4g/ml and 0.2g/ml significantly reduced (P lt; 0.01); When drug concentration 0.1g/ml, the decrease in the secretion of VEGF (P lt; 0.05); the inhibitor group on the secretion of VEGF compared with the control group no significant differences (P gt; 0.05). Conclusions: 1. Soothing itching milk in a certain concentration range inhibit keratinocyte cell proliferation, inhibition at him tazarotene no difference. Skin itching milk may be one of the mechanisms for the treatment of psoriasis, which play a role in inhibiting keratinocyte cell proliferation by inhibiting the activation of p38 MAPK signal channel. 3. Soothing itching milk can reduce the secretion of VEGF, but its secretion has nothing to do with the activation of p38 MAPK signal channel.

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