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Objective: To construct PCO rat model to investigate the the kidney circulation Treatment of the mechanism and significance of polycystic ovary syndrome by observing the impact of of Bushen Huayu party PCO rat ovarian granulosa cells on ER and AR gene expression . Methods: 79 6 - week-old SPF level regular estrous cycle of the female SD rats were randomly divided into normal group and model group 1:5 : normal group with 1% carboxymethyl cellulose aqueous solution orally for 21 days ; made ??module with the preparation of letrozole - carboxymethyl cellulose solution orally induced rat model of polycystic ovarian pathology . After the modeling , the model group were randomly divided into : ① model the control group (n = 12 ) , saline ( qd x 3d, rest 1d ) × 21 times ; ② kidney Huayu Fang high dose group ( 13 ) , high-dose gavage Bushen Huayu Recipe , ( qd x 3d, rest 1d ) × 21 times : ③ Bushen Huayu Fang dose group ( 13 ) , kidney circulation prescription dose orally ( qd × 3d, rest 1d ) 21 times ; ( 4 ) Bushen Huayu Recipe low-dose group ( 13 ) , Bushen Huayu Recipe low-dose orally ( qd x 3d, rest 1d ) 21 times ; the ⑤ positive drug control group ( 13 ) , up to -35 solution orally ( Qd × 3D rest 1d ) 21 times . After the end of the intervention period , each randomly selected eight rats with one ovary separation granulosa cells , real-time PCR determination of gene expression values ??Bushen Huayu Recipe PCO rat ovarian granulosa cells ER and AR expression . Results : ( 1 ) the evaluation of PCO rat model : rat estrous cycle loss of regular changes , has been in diestrus , suggesting that ovulation ; under the light microscope model rats the ovarian form a multi- cystic changes ; , model group, serum LH , T levels were significantly higher than the normal group ( P lt ; 0.01 ) , E2 levels were significantly lower than normal group ( P lt ; 0.05 ) ② PCO rat ovarian granulosa cells ER , AR gene expression : made ??module ER , AR gene level was significantly lower than normal group ( P < 0.01 ) ; application Bushen Huayu Recipe after the intervention , high and low dose group ER , AR was significantly higher the model control group ( P lt ; 0.05 ) . Conclusion : ① Application letrozole prepared PCO rat model is an ideal model with PCOS , reliability strong . The ② Bushen Huayu Recipe can be raised PCO rat ovarian granulosa cells on the level of gene expression of ER and AR .
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