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Objective To study the main pharmacological ingredient of traditional Chinese medicine in China Ginseng Leaves ginsenosides whether there is a protective effect on acute lung injury in rat lung tissue, and to explore the possible mechanism of action. Methods 40 SD rats were randomly divided into four groups: control group, model group, ginsenosides (GS) group, dexamethasone group. Groups of drugs from the tail vein injection, after the start of the experiment, the control group and model group were injected with normal saline (NS) 1ml GS group were injected with the GS a solution 100mg/kg, dexamethasone group were injected with dexamethasone liquid 5mg/kg. After 1 hour, the control group injected 1ml NS, model group, GS group and dexamethasone group were injected endotoxin to 5mg/kg; observe the general situation of the rat after 6 hours; abdominal aortic blood line blood gas analysis; Remove the left lung, said the wet weight, dry weight, calculated lung coefficient (wet / dry weight ratio); take the right lung general observation with the naked eye and do a biopsy 200 times the light microscope; lavage fluid and measurement of SP-A protein and the content of the SP-B protein. Results of the experiment 40 rats without loss and death, its all the results of analysis. General condition: the rats drugs all injection completed after 6h, the respiratory rate of the control group :56-64 beats / min, no abnormal signs. Respiratory rate of the rest of the rats: model group :121-134 times / min, the ginsenosides group :95-106 beats / min, the dexamethasone group of 83 - 91 beats / min, there are varying degrees of respiratory distress , limbs cyanosis. Lungs naked eye general observation: the control group, the lung surface was smooth like no abnormal changes; model group color dark red, a large bleeding point and infarction; the dexamethasone group slightly edema and a small amount of bleeding Point; saponin group congestive edema more obvious, visible bleeding. 4.200 times the light microscope: a clear structure of the lung tissue of the control group, no special change; model group, the tiny blood vessels congestion, exudation. The pulmonary interstitial and alveolar spaces a lot of the liquid exudation and inflammatory cell infiltration, can see spotty atelectasis; ginsenoside group seeping light compared with the model group; tiny the dexamethasone group of lung tissue vascular congestion pulmonary interstitial and alveolar space, there is a small amount of inflammatory cell infiltration. Arterial blood gas: Compared with the control group, and the remaining rats PaO2 and PaO2/FiO2 decreased significantly (P lt; 0.01); injury group compared with the saponin group, dexamethasone group relatively higher (P lt; 0.01) ; compared with saponin group, dexamethasone group slightly higher (P lt; 0.05); 6. compared with the control group, the other three groups of SP-A protein and SP-B protein content were lower (P lt; 0.01); compared with the model group, the relatively high the two protein content of saponin group and dexamethasone group (P lt; 0.01); saponin group, slightly higher the two protein content of the dexamethasone group (P lt; 0.05). Conclusion 1. Ginsenosides solution can mitigate acute lung injury in rats respiratory distress, improve ventilation and reduce pulmonary edema. 2 ginsenosides solution is reduced by inhibition of rat lung tissue protein SP-A and SP-B protein content to treat acute lung injury.
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