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The periarthritis referred to as frozen shoulder, the Chinese medicine the periarthritis called \Frozen shoulder around the shoulder muscles, ligaments, tendons, bursa, joint capsule and other soft tissue damage, degenerative joint capsule and articular soft tissue caused by a chronic aseptic inflammation. The clinical manifestations of shoulder pain, shoulder mobility, especially in the affected arm outreach, external rotation, and the most obvious extension, long time, visible shoulder muscle atrophy, adhesions, belongs to Chinese medicine \The aim of the present study was acupotomology interventions, through the organization of morphological observation and the use of molecular biology techniques, the analysis of related substances of tendon collagen scar formation, reveal needle knife in Treating role in the metabolism of collagen tissue mechanism, provide for the frozen shoulder acupotomology clinical experimental basis. Methods 48 healthy male New Zealand rabbits were randomly divided into 6 groups, respectively, for the normal group (control group), model group, EA group, knife group A (duration of pain intervention), the knife group B (period of stiff intervention ), the the knife group C (pain and stiff period of co-intervention) 8 each. The application continued strain plus the ice the prepared frozen shoulder rabbits model. Normal group (control group), model group, non-intervention, needle-knife set of rows acupuncture therapy, electro-acupuncture group line EA treatment, each group reared under the same conditions, a total of 33 days. Were taken biceps, supraspinatus muscle, infraspinatus tendon, Determination of hydroxyproline and total protein content. Data processing using the SPSS 17.0 statistical software for analysis and testing. Determination of tissue hydroxyproline content, sample alkalinehydrolysis for UV absorption determination of total tissue protein content. 1 local histopathological observation: blank group tendon adventitial connective tissue surrounding rows of 1-2 layers of cuboidal cells, the outermost layer of epithelial cells, tendon fibers arranged in neat rows; model group tendon outer membrane of epithelial cells up to 4-5 layers, disarranged, increased the epithelium deep downlink fiber, dense structure; basically the same pathological changes of the EA group and needle-knife treatment group, tendon outer membrane of epithelial cell proliferation, but less than the model group, mostly 2-3 layers on the subcutaneous connective tissue is denser. Hydroxyproline in the biceps, supraspinatus muscle, The infraspinatus tendon Determination results show that after the success of the model, the model measured results significantly higher than the other groups, and each group has a very significant differences (P lt; 0.01). Between the control group and EA group and the acupuncture therapy group did not differ (P gt; 0.05). Hydroxyproline measured in different tendon the content overall trend also more consistent. 3, biceps, supraspinatus, infraspinatus muscle tendon in total protein content measured results show blank group, the total protein content is lower than other groups level, the blank group acupotomology A, B group differences significant (P lt; 0.05), acupotomology A group B was no difference (P gt; 0.05). Conclusions local organizations HE staining showed that acupuncture therapy can be effectively reduced lesion collagen scar tissue formation. Knife treatment, the muscle fibers arranged in neat rows, acupuncture therapy not only can effectively prevent pathological scar formation, but also restore the local static and dynamic equilibrium state, the activities of the organization does not affect recovery of muscle function. Local tissue scar direct acupotomology the shovel set aside, cutting related. 2 needle knife hydroxyproline and protein content of frozen shoulder model rabbit tendon a benign regulatory role. In the early and mid-term tissue repair, promote collagen secretion by acupotomology intervention to accelerate tissue repair process, and in the tissue repair the late acupotomology intervention pathological scar formation. 3 The experimental set the contrast of the needle-knife and electro-acupuncture treatment. From the formation of collagen scar, EA group and acupotomology each treatment group had similar effects, hydroxyproline and protein content alone can not determine the advantages and disadvantages of these two therapies. However, the number of accupotomology repair and can prevent excessive formation of tissue adhesions contracture clinical soft tissue injury treatment or guidance.
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