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Target gene in plant genetic transformation with multiple selectable marker plant expression vector infected plant selectable marker on the carrier detection is to monitor the functional gene diversity of crop varieties and applicability of the common practice. In this study, the plant expression vector pCAMBIA1301 receptor gene of the the cloned exogenous fragment soluble redshift of green fluorescent protein (smRSGFP), anti-herbicide Basta selection marker bar gene and its downstream of a plant the CaMV35S sub this carrier, build out the containing smRSGFP, multi-selection marker carrier bar, the enzyme of the β-glucuronidase (GUS) and tidal neomycin (HPT) resistance gene. Then cloned from maize small heat shock protein (sHsp) connected to the bar gene downstream of CaMV35S promoter, the carrier containing four marker genes, also carrying a functional gene. Only detected smRSGFP gene in Escherichia coli, Agrobacterium, still corn preliminary detection of gene expression of various marker genes and sHsp. In this study, the following conclusions: (1) The green fluorescent protein gene construct this carrier, the carrier pCAMBIA1301-smRSGFP in E. coli DH5α and Agrobacterium LBA4404, corn Backbone Parental Chang 7-2, groups of inbred lines YQ7-96 were able to express a green fluorescent; (2) herbicide resistance gene bar to import vector pCAMBIA 1301-smRSGFP vector pCAMBIA1301-smRSGFP-bar to go to Agrobacterium infection corn Chang 7-2, YQ7-96, with the tide neomycin and herbicides were screened corn to the survival of plants, the bar and hygromycin-resistant gene vector can be expressed in maize; (3) vector pCAMBIA1301-smRSGFP-bar carrier to continue to add functional gene sHsp corn Chang 7-2 YQ7-96 thermal stress and GUS staining screening show that these two genes in this carrier smooth expression. The success of this vector construct multiple gene screening test multi-markers carrier, the screening test, with simplicity and broad applicability. Multi-selective marker not only can reduce the chance of false-positive plant genetic transformation, also can be screened according to the conditions and needs of specific experimental detection, thus effectively reducing the screening costs and save the screening time. In the same time, this carrier can not only does not destroy the the carrier characteristics premise, to import bar gene downstream of CaMV35S promoter connect detect target genes, but also under the same promoter of target genes and fluorescent gene fusion expression, while for each marker genes characterized by the spirit of efficient, simple, easy principle to take the detection method has a strong originality. The sHsp-containing gene vector was successfully constructed and detection, detection of marker gene carrier has the effect not only makes verify verify a functional gene. The carrier above advantages are conducive to the carrier in the specific practice widely.
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