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Capillary electrophoresis (Capillary Electrophoresis, CE) is an ionic or charged particles in high electric field as the driving force, in the capillary in accordance with the sample between the mobilities of the components and / or the difference in distribution coefficient for the efficient and rapid separation A new liquid separation technology. Capillary electrophoresis is an efficient, fast, small amount of sample, easily automated, easy to operate, less solvent consumption, environmental pollution, etc., its research and has widespread applications in analytical chemistry, biomedical, food science, environmental science and other fields, separation and analysis of science has become the most dynamic and promising research methods. Is used in combination with capillary electrophoresis There are many types of detectors, such as optical, electrochemical, mass spectrometry detectors, etc., while the electrochemical detection of amperometric detection technology is our main research group detection method has high sensitivity, detection volume small, wide linear range, simple operation, low cost instrument, thus capillary electrophoresis - amperometric detection hyphenated techniques in analytical chemistry and other fields have been increasingly extensive research, application and development. This thesis is based on capillary electrophoresis - amperometric detection technique is rapid, sensitive, efficient, etc., on the Chrysanthemum in flavonoids and anthraquinones and blueberry wine in the six active substances simultaneous separation and determination to expand the application of capillary electrophoresis range. This thesis is divided into the following three parts: one, a brief overview of the historical development of capillary electrophoresis, the basic principle, the injection mode, split mode, the detection combined with technology, research and application, as well as the purpose and significance of thesis . 2, using capillary electrophoresis - amperometric detection (CE-AD), on Chrysanthemum in flavonoids and anthraquinones (emodin, kaempferol, apigenin, luteolin and Rhein) for the separation and determination. Working electrode potentials were investigated, running buffer pH and concentration, separation voltage and injection time experimental parameters on the experimental results. Under the optimum conditions, with a diameter of 300μm carbon disk electrode as working electrode, a detection potential of 0.90 V (vs. SCE), separation voltage was 19 kV, in 15 mmol / L borax-30mmol / L potassium dihydrogen phosphate (pH 9.0) running buffer solution, five analytes can be achieved within 17 min in a good baseline separation of analyte concentration and peak currents showed good linearity, limit of detection (S / N = 3) ranging from 1.0 × 10-7 to 2.1 × 10-7g/mL. The method has good reproducibility, has been applied to the analysis of four kinds of chrysanthemum samples, the sample is simple, without pre-enrichment obtained satisfactory results. 3, using capillary electrophoresis - amperometric detection (CE-AD), the first time the active substance blueberry wine (kaempferol, ferulic acid, vanillic acid, caffeic acid, gallic acid and protocatechuic) were isolated and determination. Working electrode potentials were investigated, running buffer pH and concentration, separation voltage and injection time experimental parameters on the experimental results. Under the optimum conditions, the borax - potassium dihydrogen phosphate (pH 7.8) running buffer solution, the analyte can be achieved within 18 min in a good baseline separation. Detect potential of ten 0.95 V (vs.SCE), the measured object 300μm in diameter carbon disk working electrode has good current response, the concentration and the peak current in the three orders of magnitude showed good linearity, limit of detection ( S / N = 3) up 10-8g/mL, recoveries ranging from 95.8% -106.7%. The method has been successfully applied to the analysis of real samples obtained satisfactory results, for the wine used in the product quality control.
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