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Objective To observe the Severe Acute tumor necrosis factor-a (tumour necrosis factor-a, TNF-a), diamine oxidase (diamine oxidase, DAO), tight junction protein -1 (zonula occludens-1, ZO-1 ) expression levels and their relationship with the intestinal barrier damage explore Ulinastatin intervention after the change. Methods 60 male SD rats were randomly divided into sham operation (sham operation, SO) group, severe acute pancreatitis (severe acute pancreatitis, SAP) group and ulinastatin (ulinastatin, UTI) treatment groups, each animal was randomly divided 6,24 h 2 time points, each time point 10. Retrograde cholangiopancreatography puncture injection of 5% sodium taurodeoxycholate SAP model, respectively after modeling 6,24 h rats were sacrificed. Modeled after the success observed in serum TNF-a levels and DAO activity, while observing the pancreas and ileum pathological changes were protein localization by immunohistochemistry and RT-PCR detection of ileal tissue ZO-1 protein and mRNA expression levels. Results SO group, SAP group, UTI treatment group 6h serum TNF-a were (10.83 ± 0.96) ng / L, (181.89 ± 4.93) ng / L, (128.23 ± 2.40) ng / L, 24h serum TNF-a, respectively, was (9.89 ± 0.14) ng / L, (198.89 ± 4.83) ng / L, (106.79 ± 3.30) ng / L, differences between the groups were significantly (P lt; 0.05); 6h plasma DAO activity were (354.79 ± 3.67 ) U / L, (117.21 ± 5.58) U / L, (282.98 ± 9.12) U / L, 24 h plasma DAO activity were (313.36 ± 4.43) U / L, (91.18 ± 1.09) U / L, (234.11 ± 8.89) U / L, differences between the groups were significantly (P lt; 0.05); 6h ileum ZO-1 protein expression was 10.00 ± 1.87 points, 1.20 ± 0.84 points, 5.80 ± 2.86 points, 24 h ileal tissue ZO- 1 protein expression was 8.60 ± 2.19 points, 0.80 ± 0.84 points, 2.40 ± 1.14 points, differences between the groups were significantly (P lt; 0.05); 6h ileum ZO-1mRNA expression was 0.8779 ± 0.0148,0.4660 ± 0.0230,0.7780 ± 0.0327,24 h ileal tissue ZO-1mRNA expression was 0.8300 ± 0.0235,0.5502 ± 0.0271,0.7040 ± 0.0114, differences between the groups were significantly (P lt; 0.05). SAP6h, 24h two subgroups of serum TNF-a levels than the SO group was significantly higher (P lt; 0.05), plasma DAO activity, ileum ZO-1mRNA and protein expression compared with SO group was significantly lower (P lt; 0.05), while the pancreas organizations and ileal mucosal villous epithelial necrosis, vascular hemorrhage, inflammatory cell infiltration; UTI treatment group 6h, 24h two subgroups of serum TNF-a than SAP group was significantly lower (P lt; 0.05), plasma DAO activity, ileum ZO-1mRNA and protein expression was significantly increased compared with SAP group (P lt; 0.05), while pancreatic tissue and ileal mucosal villous epithelial necrosis, vascular hemorrhage, inflammatory cell infiltration was reduced. Conclusion ileum ZO-1 downregulation of SAP intestinal barrier injury is one important reason may be related to inflammatory cytokines TNF-a and the excessive release of the decreased plasma DAO activity. Ulinastatin may inhibit the excessive release of TNF-a, increase in plasma DAO activity, which increased ileal tissue ZO-1 protein and mRNA expression, protect the intestinal barrier.
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