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Experimental Objective: Apply artificial hypobaric chamber simulating altitude training methods, to Lanzhou anchored 1500m above sea level, the establishment of animal training alternating gradient elevation model to investigate the fixed altitude of 2500m, 4000m and 2500m ~ 4000m above sea level alternately hypoxic training on liver antioxidant capacity, the ability of anti-injury, as well as indicators of hepatic energy metabolism, c-fos gene expression in order for athletes training alternating high - high to provide a reference. Experimental Methods: In healthy male Wister rats as research object, Treadmill training methods to establish animal training model. The treadmill habilitation, filtered rats were randomly divided into: normoxic quiet group (NQ), normoxic training group (NT), 2500m altitude hypoxia training group (2500T), 4000m altitude hypoxia training group (4000T) elevation of 2500m and 4000m alternating hypoxic training group (AHT). Training group (NT, 2500T, AHT, 4000T) rats incremental continuous training four weeks, training six days a week, rest one day. Training two weeks after the training group were randomly selected six, respectively, in order to 25m/min speed treadmill Exhaustive instantly decapitated, the livers were fixed in formalin, detect c-fos gene expression in the liver. Other rats completed four weeks after training normoxic resume training four days, respectively, in the first five days to 25m/min speed treadmill Exhaustive instantly decapitated, recording exhaustive time, each group were detected in rat liver glycogen, MDA content, liver SOD, GSH-PX, CAT, SDH, ATP activity, c-fos gene expression, blood BUN levels, ALT, AST activity indicators different variations. NQ rats do not participate in sports, along with training four weeks rats were decapitated, the liver immediately detect c-fos gene expression in the liver. The experimental results showed that: (1) alternating hypoxic training can significantly prolong rat treadmill time to exhaustion and improve endurance. (2) alternating hypoxic training group rat liver GSH-PX, CAT activity than the other groups, indicating that the liver cells alternately hypoxic training group free radical scavenging ability; hypoxic training group than in rat liver MDA content normoxic training group, which may be due to hypoxia training rats after exhaustive exercise when liver metabolism, free radicals produce higher, although the free radical scavenging capacity is improved, but there is still a certain amount of free radicals accumulate, leading to liver lipid peroxidation compared with normoxic training group increased. (3) in the blood of rats hypoxic training ALT, AST activity than normoxic training group, indicating that hypoxic training improves the liver's ability to resist damage. (4) alternating hypoxic training group rat liver Na -K -ATP, Ca 2 -ATP, SDH enzyme activity than other group, indicating liver aerobic activity increased. (5) alternately hypoxic training can effectively improve liver glycogen reserve, for the movement to provide more energy. (6) training for two weeks each rat liver was no difference in c-fos expression, and training around the rats trained for two weeks than the c-fos expression in rat liver decreased, alternating hypoxic training group c-fos expression lower than other groups, which may be associated with liver cell adaptability, ability to raise anti-stress related. Conclusion: After four weeks simulated hypoxic training alternating gradient altitude normoxic resume training after four days then moderate intensity exercise to exhaustion, analyzing the rats indicators show that the simulated altitude of 2500m ~ 4000m alternating hypoxic training relatively suitable height and load , can significantly improve the rat liver GSH-PX, CAT, Na -K -ATP, Ca 2 -ATP, SDH activity and liver glycogen reserve, but significantly lower than other groups in blood ALT, AST activity levels, and liver cells c-fos expression, which to some extent, improve the anti-oxidation, anti-damage and aerobic capacity, improve rat liver ability to adapt to moderate-intensity exercise.
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