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Objective: To study intratracheal injection of amikacin tracheal mucosa of Wistar rats ultrastructure and pathological effects, and using electron microscopy and light microscopy were observed ambroxol intervention after tracheal mucosa of rats to amikacin changes the impact of a better understanding of expectorant drug ambroxol on mucociliary system in rats. MATERIALS AND METHODS: A total of 120 male Wistar rats were randomly divided into four groups: normal control group, saline group, amikacin group, the intervention group ambroxol 30. Amikacin rats after intratracheal injection of anesthetic doses of amikacin corresponding saline group injected with normal saline, ambroxol intervention group were injected with amikacin 6h before I Ambroxol 70 mg / kg orally, then injected dose per body weight such as amikacin, normal control group of normal feeding not inject drugs. Were 2,12,24,48,72 hours after administration, rats were sacrificed dissecting the trachea, scanning electron microscopy ultrastructural changes of bronchial mucosa with Image-Pro Plus 6.0 image analysis software for each scanning electron microscopy Picture semi-quantitative analysis of cilia damaged area, and observed changes in lung tissue pathology. Result: an electron microscope, saline group and amikacin rats tracheal mucosa cilia are different degrees of damage, manifested as disorganized, lodging, adhesion, shorter, part of the fracture missing, with varying degrees of mucus secretion increases. Light microscope amikacin rats lungs cilia disorders, mucus secretion, goblet cells increased, partly seen in bronchopulmonary tissue infiltration of inflammatory cells. 2, the saline group, amikacin group, ambroxol intervention group were cilia damaged area than the normal control group increased (P lt; 0.01). 3, in each respective time point, amikacin group cilia damaged area greater than saline group and ambroxol intervention group (P lt; 0.01); ambroxol amikacin was injected after intervention by the cilia extent of damage has been reduced compared with amikacin group (P lt; 0.01), cilia structure better recovery. 4, amikacin group damaged area comparison group, the first 2h significantly higher than the first 12 (hP lt; 0.01), greater than the first section 12h 24h (P lt; 0.01), the first 24h higher than the first 48 (hP lt ; 0.01), significantly higher than the first 48h 72h (P lt; 0.01); ambroxol intervention group compared damaged area, the first 2h and 12h compared to although decreasing trend, but not statistically significant (P gt; 0.05), and significantly higher than the first 12h 24h (P lt; 0.01), the first 24h and 48h damaged area showed no significant difference (P gt; 0.05), significantly higher than the first 48h 72h (P lt; 0.01). 5, three groups of cilia damaged area are compared between the two groups, the first group of damaged area greater than 2h amikacin ambroxol intervention group, ambroxol intervention group than the saline group (P lt; 0.01); first 12h, 24h amikacin group ambroxol damaged area is larger than the intervention group (P lt; 0.01), but ambroxol intervention group and the saline group had no significant difference (P gt; 0.05); Article 48h, 72h amikacin group was significantly larger than the damaged area ambroxol intervention group, ambroxol intervention group than in the saline group (P lt; 0.01). Conclusions: 1, intratracheal saline injection, amikacin, may on the surface of rat tracheal mucosa and lung bronchioles ciliary ultrastructure cause varying degrees of acute mucosal damage, but the damage is reversible, increases with time self- repaired. 2, after the intervention of ambroxol in rats by intratracheal instillation of amikacin Although cilia structural damage, but the extent of amikacin group compared with mild lung pathology prompted a rare inflammatory cells, suggesting that ambroxol can promote cilia structure repair, and to reduce lung inflammation.
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