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Rifampin sodium alginate microspheres embolic agents in vitro release properties of

Author: YangLiNa
Tutor: ZuoTingZuo
School: Chinese Pharmaceutical and Biological Products
Course: Drug analysis
Keywords: Sodium alginate microspheres embolism agent In vitro release Stability
CLC: R943
Type: Master's thesis
Year: 2011
Downloads: 52
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Abstract

Objective: To investigate rifampicin sodium alginate microspheres vascular embolic agents in vitro under different environmental release behavior and study the formulation of the drug content, stability, degradation behavior in the release medium, so the products do preliminary evaluation of clinical trials and to provide reference. Methods: observed with a scanning electron microscope (SEM) rifampicin the alginate microsphere appearance and its internal structure; were studied by high performance liquid chromatography (HPLC) of the formulation of the drug content, stability. Study rifampicin alginate microspheres vascular embolic agent in the release medium of the different concentration of BSA (bovine serum albumin), the different volumes of the release medium and the release behavior of the different shaker speed thereby examine the release behavior of the formulation, Also, a preliminary study on the degradation behavior of the drug. Results: Scanning electron microscope rifampicin sodium alginate microspheres rough surface, irregular protrusions, the internal structure of loose, porous. Rifampicin liquid phase conditions optimized mobile phase each match ratio of methanol: acetonitrile: 0.075 mol / L potassium dihydrogen phosphate solution: 1.0 mol / L citrate solution (30:32:36:4) column temperature was 35 ℃, containing the test results of the dose: low-dose (100 ± 10 mg / g), medium dose (200 ± 10 mg / g), high-dose (400 ± 10 mg / g) of rifampicin alginate microspheres containing drug loading were 178.7024 ± 9.0310mg / g, 282.8304 ± 5.3136 mg / g, 387.6569 ± 15.5793 mg / g. Rifampicin alginate microspheres in vitro release test results: With the release of the increase of the concentration of the medium, the maximum cumulative release rates of the microspheres increased; released in the different volumes of the release medium, the maximum cumulative release rate as volume increases, but the maximum cumulative release rate as compared with the release of 30 mL and 50 mL in 10 mL of release medium in the significant difference; shaker speed is not the same, the rotational speed increases, the maximum cumulative release rate increases. Rifampicin reference substance degradation in the release medium from medium concentration of BSA and shaking speed, affected by the volume of the release medium, the release accelerate the increase of medium volume. Conclusion: rifampin sodium alginate microspheres rough surface, the internal pore structure visible in the scanning electron microscope, this structure is conducive to the release medium infiltration of drug dissolved released; drug content, the high-dose group micro ball drug content meets the labeled amount, but the low-dose group and middle dose group microspheres drug content of the labeled amount quite different; different concentrations, the volume of the release medium and shaking speed rifampicin sodium alginate microspheres release some impact, but overall showed the the microsphere drug release performance; rifampicin instability in the release medium, readily biodegradable and analysis should be considered during the release of the test results.

Rifampin sodium alginate microspheres embolic agents in vitro release properties of

Abstract

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