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Empirical Study on the Adrenomedullin(ADM) Expression in Renal Cell Carcinoma by the Low Oxygen Factors

Author: WangZuo
Tutor: YangShuWen
School: Hebei Medical University
Course: Surgery
Keywords: Kidney Cancer Hypoxia ADM Western blot
CLC: R737.11
Type: Master's thesis
Year: 2011
Downloads: 3
Quote: 0
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Abstract


Objective: In China, renal cell carcinoma is a serious threat to people's lives and health of one of the diseases, the most common tumors of the urinary system. In recent years, ADM in the incidence of ovarian cancer, lung cancer and other malignancies, the development has been growing attention to its role in renal cell carcinoma has also begun to receive widespread attention and research. Adrenomedullin (Adrenomedullin ADM) initially in 1993 by Japanese scholars from people addicted pheochromocytoma isolated a vasodilator peptide composed of 52 amino acids, and calcitonin gene-related peptide (Calcitonin-gene related The homology between the peptide, CGRP) was 24%, and having a a typical disulfide bridge, belonging to the family of CGRP. Clinical studies have shown that The ADM is overexpressed in many types of cancer patients, and was positively correlated with the histological grade and malignant degree, and is associated with a poor prognosis. Many complex biological effects in the body can play. Activate adenylyl cyclase-PKA signaling pathway, the intracellular cAMP levels; increase intracellular calcium concentration induced vasodilation and activate the endothelial cells of nitric oxide (NO) synthase; activation of MAPK signal transduction pathway to promote cell mitosis of staff may be closely related with tumor development. Hypoxia is a common feature of solid tumor growth have hypoxia stimulate tumor cells to produce a series of reactions to promote tumor growth and progress. Studies have shown that hypoxia can stimulate the expression of ADM in the organization and subsequently there are many reported a variety of tumor cells in a hypoxic environment ADM overexpression. The hypoxic environment ADM overexpressed then exert their biological effects and plays an essential role in the progression of tumor growth. Human renal cancer, given the observation of ADM expression levels related to hypoxic stress treatment, hypoxia factors of ADM further verify RCC. At home and abroad for renal cell carcinoma in ADM less a result of this study is to verify and explore development mechanism and renal cell kidney cancer may occur clinical diagnosis and reasonable treatment to provide a theoretical basis. Methods: 1 material: human renal carcinoma cell line 786-O, purchase by the Beijing Union Medical College Institute for Basic Research. 2 Method: cultured renal cell carcinoma cells, and were given the different conditions of hypoxic environmental stimuli, using Western blotting (Western blot) to detect the level of expression of each group of renal cell carcinoma cells of ADM. 3 application SPSS13.0 software for statistical processing results. ADM protein expression levels using independent samples T-test method or variance analysis test method. Test level of 0.05. The result: the various conditions of hypoxia stimulate ADM protein levels βactin protein: a molecular weight of 42 kD 42kD position Western After hybridization, a positive bands. The hybridizing bands semi-quantitative analysis using gel image analysis system, the protein content of the integrated optical density (IOD). Each time the to detect protein are detect βactin protein, as the standard ADM protein detected by quantitative. ADM protein: molecular weight 7kD Western after hybridization positive band 7kD position. Hybridizing bands semi-quantitative analysis using gel image analysis system, protein content IOD relative integral optical density protein expression level. Cells compared with the control group, the cell lines of various conditions of hypoxia stimulation group have different levels of ADM high expression was positively correlated with the stimulation time. Conclusion: hypoxic environment can promote kidney cancer cells in the expression of ADM. However, the specific biological effects of hypoxia environment for the the specific mechanism ADM ADM expression in renal cell need to further explore.

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CLC: > Medicine, health > Oncology > Genitourinary tumors > Urinary tumors > Kidney,renal pelvis tumor
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