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Objective: To study the ATP-binding cassette transporter -1 (ATP-binding cassette transporter 1, ABCA1) I883M single nucleotide polymorphisms (single nucletide polymorphisms, SNPs), and 3 - hydroxy-3 - methylglutaryl coenzyme A ( 3-hydroxy-3-methylglutaryl coenzyme A, HMG-CoA) reductase gene ScrF 1 restriction site polymorphism and cerebral infarction (cerebral infarction, CI) correlation between the gene polymorphisms were observed compared susceptibility of cerebral infarction relationship. And the risk factors for cerebral infarction observed in order to further investigate the pathogenesis of cerebral infarction. And further in-depth study of cerebral infarction and preventive mechanisms. Methods: Polymerase chain reaction (polymerase chain reaction, PCR) technique and restriction fragment length polymorphism (restriction fragment length polymorphism, RFLP) analysis of 90 cases confirmed by CT or MRI in patients with cerebral infarction and 100 patients did not people suffering from cerebral infarction control group case-control study, the application of PCR - RFLP technique ATP-binding cassette transporter 1 gene I883M single nucleotide polymorphisms, and 3 - hydroxy-3 - methylglutaryl coenzyme A reductase ScrF 1 enzyme gene polymorphism loci analyzed, and detection and analysis SPSS13.0 statistical package for data applications gene genotype and allele frequency distribution of the comparison, and thus a target gene can be observed multiple polymorphic loci Normality and the relationship between cerebral infarction and preventive mechanisms for the further study of gene basis. Results: 1 Application chi-square test for cerebral infarction group and non-group population distribution of each gene Hardy-Weinber genetic equilibrium test (P gt; 0.05) are shown in Table 1, the distribution of genotypes in each group are in line with the genetic balance, The study population has group representation. 2 non-cerebral infarction group and the control group of people, ABCA1 gene I883M polymorphism various genotypes (P = 0.183) and allele frequency (P = 0.916) had no significant difference in the distribution of cerebral infarction group I883M 883I polymorphism allele frequency slightly higher than the non-cerebral crowd control group (74%, 61.8%), that the 883I allele may be associated with the pathogenesis of cerebral infarction, but not statistically significant (p = 0.13), subject to further expand the crowd, further confirmed. Three non-cerebral infarction group and the control group of people, ABCA1 gene I883M polymorphism various genotypes (P = 0.183) blood high population density lipoprotein no significant difference, because the two groups of patients showed a high density lipoprotein non-normal distribution, the application of two non-parametric test for independent samples analyzed two groups of high-density lipoprotein distribution. 883I gene carriers high-density lipoprotein levels (1.39 ± 0.52) is slightly lower than non-883I gene carriers (1.46 ± 0.53). But not statistically significant (p = 0.925), to be further confirmed their relationship. Four pairs of non-cerebral infarction group of people with the control group, ABCA1 gene I883M genotype II polymorphic loci percentage slightly higher than non-cerebral crowd control group (8.9%, 4%), and brain may genotype II infarction morbidity related, but not statistically significant (p = 0.234), need to further expand the crowd confirmed. 5 pairs of non-cerebral infarction group and crowd control group, HMG-COA reductase gene ScrF Ⅰ restriction sites A allele frequency slightly higher than the non-cerebral crowd control group (51.7%, 49.5%), possibly A allele was associated with the pathogenesis of cerebral infarction, but not statistically significant (p = 0.682), may be related to insufficient number of cases, the need to expand the number of cases further confirmed. 6 non-cerebral infarction group of people with the control group, HMG-COA reductase gene ScrF Ⅰ restriction site polymorphism various genotypes (P = 0.867) and allele frequency (P = 0.682) no significant difference in the distribution of cerebral infarction group ScrF Ⅰ restriction sites A single nucleotide polymorphism allele frequency slightly higher than non-cerebral crowd control group (51.7%, 49.5%), but no statistically meaning, to be further confirmed. 7 groups of several blood biochemical test results and gender, age, smoking history, alcohol history and other risk factors for comparison drawn: Age (p = 0.027), triglycerides (P lt; 0.001), glucose (p = 0.003) , high-density lipoprotein (P lt; 0.001), hypertension (P lt; 0.001), history of alcohol consumption (p = 0.05), smoking history (p = 0.025), there were significant differences in the total cholesterol (p = 0.208 ), gender (p = 0.658) between the two groups were not statistically significant. 8 The ABCA1 gene I883M polymorphisms and HMG-COA reductase gene ScrF Ⅰ restriction sites, triglycerides (triglyceride, TG), age, gender, plasma total cholesterol (Total Cholesterol, TC), blood sugar (glucose, GLU), high density lipoprotein (high-density lipoprotein, HDL), past history and history of alcohol and other possible risk factors for stroke include, Logistic regression analysis, were not included in the regression equation. Conclusions: In Hebei Han population: 1 ABCA1 I883M polymorphic loci 883I with cerebral infarction may be relevant. 2 HMG-CoA reductase gene ScrF 1 polymorphisms and cerebral infarction was no significant correlation. Three patients with various risk factors compared with cerebral infarction, age (p = 0.027), triglycerides (P lt; 0.001), glucose (p = 0.003), high density lipoprotein (P lt; 0.001), hypertension ( P lt; 0.001), history of alcohol consumption (p = 0.05), smoking history (p = 0.025), may be associated with the occurrence of cerebral infarction, and total cholesterol (p = 0.208), gender (p = 0.658) between the two groups without significant difference in the incidence of cerebral infarction may be irrelevant. 4 pairs of various risk factors for cerebral infarction Application Binary Logistic regression analysis, the risk factors were not included in the regression equation, all patients with risk factors may lead to cerebral infarction together.
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