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The diethylstilbestrol and double-ene female phenol is structurally similar synthetic non-steroidal estrogen phenolic hormone for estrogen supplement clinical treatment of breast cancer, prostate cancer, amenorrhea and menopause syndrome, and to enable women the normal development of organs and secondary sex characteristics, prompting endometrial hyperplasia, enhanced uterine contractions, uterine oxytocin sensitivity; small doses to stimulate the anterior pituitary secretion of sex hormones and prolactin, large doses depress. The diene female phenolic metabolites of diethylstilbestrol. In animal husbandry, diethylstilbestrol and diene female phenol is mainly used for milk production, promote protein synthesis, increase protein deposition and decrease fat deposition. Long-term use of diethylstilbestrol and double-ene female phenol or eating foods containing Diethylstilbestrol and double-ene female phenol dairy or meat products cause of menopausal disorders, reduced fertility, female sexual precocity, the male feminine, and can cause women to produce breast, uterus cancer. Therefore, the establishment of a convenient, fast diethylstilbestrol and diene estrogen phenol detection methods is of great significance. In alkaline medium, Ag (Ⅲ) complex oxidation of luminol chemiluminescence signal of certain substances can significantly enhance or inhibit the chemiluminescence of the luminol-Ag (Ⅲ) system, and the luminous intensity and the concentration of the substance There was a good linear relationship within a certain range. This test Ag (III) - Luminol new system with flow injection technology combined to establish a new method of detection of diethylstilbestrol, and this method is applied to the Determination of diethylstilbestrol tablets. Capillary electrophoresis combined electrophoresis technology with modern micro-column separation technology, is the driving force Electrohydrodynamic capillary in the press the mobilities and distribution of each component behavior efficient and rapid separation. Capillary electrophoretic separation channels as a hollow capillary sample pretreatment requirements, the purpose of this study is to establish capillary electrophoresis of diethylstilbestrol and diene female hair growth products phenol. First, the flow injection-Ag (III) - Determination of the Luminol new system Diethylstilbestrol tablets of diethylstilbestrol content purposes: to establish flow injection-Ag (Ⅲ) - the Luminol new system determination of diethylstilbestrol content in diethylstilbestrol tablet new methods. Methods: under alkaline conditions, luminol oxidation of Ag (III) for generating a stable chemiluminescent signal, diethylstilbestrol can significantly inhibit the Ag (III) - luminol chemiluminescence system, and the light emitting intensity and the concentration of the sample in There was a good linear relationship within a certain range. Selection, by a series of light emitting condition of the the luminol solution concentration, alkalinity, and Ag (III) solution concentration, alkalinity, etc. to optimize the standard curve plotted under the optimum light emitting conditions diethylstilbestrol and sample content determination. Results: flow injection - chemiluminescence determination of diethylstilbestrol minimum detection limit of 3.0 × 10 -7 mol / L. The linear range of 5.0 × 10 -7 -5.0 × 10 -5 mol / L, the regression equation △ I = 5.84 × 10 8 C 548, linear correlation coefficient r = 0.9989. Continuous 1.0 × 10 -5 mol / L of Diethylstilbestrol parallel determination of seven times, the relative standard deviation of 3.4% the spiked recoveries between 88.6% -100.0%, RSD was 3.7%. Conclusion: The flow injection chemiluminescence determination of diethylstilbestrol tablets diethylstilbestrol content, the method is simple, fast, accurate and reliable, and can be used for quality control of diethylstilbestrol tablet production process diethylstilbestrol. Second, capillary electrophoresis diethylstilbestrol and diene female hair growth products phenol Objective: To establish a capillary electrophoresis - UV detection method for the determination of the new method of hair growth products diethylstilbestrol and estrogen phenol content of diene. Methods: uncoated flexible fused silica capillary (75μm id × 55 cm, effective length 50cm) for the separation of the channels, 20mmol / L borax (volume fraction of 30% N, N-dimethyl formamide) running buffer solution, using gravity injection, injection time 10s separation voltage for 20kv, UV detection wavelength of 254nm. Results: diethylstilbestrol and diene female phenol baseline separated in 9min, was a good line in the concentration range of 2.0 × 10-6-2.0 × 10-4mol / L, 2.0 × 10-6-2.0 × 10-4mol / L sexual relations, linear correlation coefficients were 0.9991,0.9994, a detection limit of 6.7 × 10 -7 mol / L and of 3.3 × -7 mol / L, recovery rates were between 89.6-94.6% and 88.7-104.8%, the relative standard deviation was 3.97% and 4.54%, respectively. Conclusion: The method is simple, rapid, accurate, commercially available hair restoration products diethylstilbestrol and double-ene female phenol detection, with satisfactory results.
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