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Bupleurum Department of Umbelliferae perennial herb, also known as Xiaochaihu, mountain vegetables, has antipyretic, anti-viral, anti-inflammatory, anti-liver injury, improve immune function and other biological activity, so it has important medical development value. This thesis raw materials, Bupleurum Bupleurum polysaccharides extraction, separation and purification, made a systematic analysis of the physical and chemical properties, structure and antioxidant activity analysis, the results are as follows: 1. Traditional hot water extraction method and ultrasonic assisted extraction to extract Bupleurum polysaccharides, respectively the Bupleurum polysaccharide WAP and SAP, the extraction rate of 2.35% and 1.73%, respectively. Manager nature of analysis, WAP and SAP containing uronic acid and impurities does not contain proteins, nucleic acids, and phenols. The environmental scanning electron microscope analysis showed that, WAP show the lamellar structure, and the SAP apparent the smaller even clastic lamellar structure, whereby the polysaccharides extracted using ultrasonic extraction, ultrasonic interested polysaccharide degradation. WAP and SAP antioxidant activity analysis, results show that measured in the reducing power, clear the O2-and anti-lipid peroxidation activity ability of SAP higher than the WAP; scavenging DPPH, WAP and SAP have high and substantially the same as the clearance rate of; WAP Clear Clear · OH capacity than the SAP. Accordingly, in the whole, the SAP antioxidant activity higher than the WAP, whereby ultrasonic polysaccharide degradation of certain effects, but did not affect the activity of the polysaccharides, but its activity increased, which may be due to degradation of the ultrasonic wave so that the the hidden reactive groups in the polysaccharide structure released. 3. The Bupleurum polysaccharide SAP quaternary ammonium fractional precipitation, and (?) Sephadex G-150 gel column chromatography separation to obtain pure product SAP1-2 and SAP3-1. Then these two polysaccharides molecular weight was determined by liquid chromatography, infrared spectrometry analysis of carbohydrate functional groups, Gas Chromatography monosaccharide composition, environmental scanning electron microscopy and atomic force microscopy polysaccharide structure, results show that the two are homogeneous polysaccharide and SAP1-2 with an average molecular weight of 3.2 × 105D SAP3-1 with an average molecular weight of 3.3 × 105D; these two polysaccharides have a distinct polysaccharide characteristic absorption peaks are pyranose and its monosaccharide residue; SAP1 -2 by rhamnose, arabinose, xylose, mannose, glucose, galactose six monosaccharide composition, wherein the molar ratio between the various monosaccharide 1.96:1.092.60:1.00:1.10:1.84 polysaccharide SAP3-1 is composed of rhamnose, xylose, mannose, glucose, galactose five monosaccharide composition, the molar ratio between the various monosaccharide is 2.07:1.34:1:1.84:1.97. Environmental scanning electron microscope analysis showed that Bupleurum polysaccharides SAP1-2 show different size sheet structure, and the surface is smooth, evenly distributed; Bupleurum polysaccharides SAP3-1 is showing the same structure as \Atomic force microscopy analysis SAP1-2 having a highly branched structure, and by the type of connection between the sugar chain to form a cyclic structure, these cyclic structure further interact to form a network structure; of SAP3-1 also has branch intertwining between the sugar chain structure and forming a cyclic structure like \4 according to the Congo red experiments, partial acid hydrolysis, periodate oxidation, Smith degradation and methylation analysis result of SAP1-2 does not have a triple helix conformation, they contain rhamnose, xylose, mannose and glucose branched polysaccharide chain wherein xylose is connected to the 1 → 2 glycosidic bond, and is connected to the higher degree of polymerization of the main chain part rhamnose is connected to a 1 → 6 glycosidic linkage, glucose, mannose and rhamnose sugar in the non-reducing end; arabinose and galactose in the main chain, and 1 → 2 or 1 → 2,1 → 6 glycosidic linkage is formed arabinogalactan. The SAP1-structure is very complex, one possible structure of the repeating units: Glc-(1 → 2) Xyl Rha-(1 → 2) Xyl 1 1 ↑ ↑ 6 6 → 1)-Gal-(2 → 1 ) Ara-(2 → 1) Gal-3 Rha-(3 → 1) Man
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