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Effect of Microenvironment of Tongue Squamous Cell Carcinoma on the Growth and Differentiation of Bone Marrow-Derived Dendritic Cell

Author: TongZuo
Tutor: LiYong
School: Chongqing Medical University
Course: Clinical Stomatology
Keywords: Tumor microenvironment Dendritic cells Immune escape Immunosuppressive factors Immune tolerance
CLC: R739.8
Type: Master's thesis
Year: 2011
Downloads: 12
Quote: 0
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Abstract


Objective: To take advantage of different doses of human tongue squamous cell Tca8113 the culture supernatants simulation tumor development impact of the tumor microenvironment of mouse marrow derived dendritic cell growth , differentiation and function of the different periods , and to explore the possible mechanism of its occurrence . METHODS: The long germ-free mice bone marrow cells in the control group in conventional recombinant mouse granulocyte - macrophage colony stimulating factor (rmGM-CSF) and recombinant mouse white interleukin -4 ( rmIL- 4 ) the culture medium culture , induced differentiate into DCs; experimental group in the culture medium , then add different doses of tongue squamous cell carcinoma cell line Tca8113 culture supernatant . Cultivate the sixth day collection of suspension cells , taking into account the living cell number of the part of the cell trypan blue staining , flow cytometry DCs phenotype (CD86, CD11c, MHC-Ⅱ) expression ; some cells to observe the exogenous addition of LPS stimuli , detected by flow cytometry after LPS stimulation the the DCs phenotype of CD86 , CD11C , MHC- II expression , mixed lymphocyte proliferation assay in vitro stimulation of T lymphocyte proliferation results : tca8113 microenvironment induced culture dendritic cells with typical morphology and phenotype ; the culture supernatants microenvironment stimulus in the low dose tca8113 enhanced stimulation of DCs mature , moderate dose of tca8113 the culture supernatant micro environment does not affect mature DCs , but can promote the differentiation of bone marrow precursor cells culture supernatants microenvironment for imDC; high dose tca8113 inhibition of DCs differentiation and maturation ; the moderate doses the cs-tca8113 microenvironment induced imDC mature ability to resist exogenous antigen stimulation . Conclusion : We successfully established model of dendritic cells induced to differentiate in tongue squamous cell microenvironment ; diversity of tongue squamous cell carcinoma microenvironment of dendritic cell differentiation may be closely related to the concentration of immune factors in the environment .

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CLC: > Medicine, health > Oncology > Oral cavity, maxillofacial tumors
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