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Objective:To evaluate the pancreatic cancer tissue Cyclooxygenase-2(COX-2)、Vascular endothelial growth factor (VEGF)、Survivin expression and its relationship with clinical pathological factors..Methods:Using immunohistochemical method (Immunchistochemistry IHC Elivision two-step)and Semi-quantitative Reverse Transcriptase-PCR technology to detect 35 cases of pancreatic carcinoma and 8 cases of normal pancreatic tissue COX-2, VEGF and Survivin expression and clinical pathological characteristics.Results:①IHC Tip:Pancreatic tissue COX-2, VEGF, Survivin expression was significantly higher than in normal pancreatic tissue (P <0.01);②pancreatic tissue COX-2, VEGF, Survivin expression and a high lymph node metastasis of pancreatic cancer a significant correlation (P <0.05);③COX-2 high expression and patient’s sex, age, tumor was no correlation between UICC stages (P>0.05), but with the VEGF, Survivin positive correlation(0<r<1, P<0.05);④VEGF, Survivin gene expression and patient’s sex, age, no correlation; with UICC tumor stage, lymph node metastasis correlation (P<0.05);RT-PCR Tip: normal pancreas group of COX-2 mRNA, VEGF mRNA, Survivin mRNA product of the electrophoretic bands dark, low output; pancreatic cancer group product band intensity, high output, the optical density compared with the normal group significantly higher (P <0.05).Conclusion:COX-2, VEGF, Survivin is highly expressed in pancreatic cancer may be related to the occurrence of pancreatic cancer, development, the prognosis, and may be closely linked to pancreatic cancer angiogene- sis;COX-2, VEGF, Survivin as pancreatic cancer is one predictor of the development.
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