|
Purpose of this study, bone marrow-derived mesenchymal stem cells (bone marrow stromal cells, BMSCs), endothelial progenitor cells (endothelial progenitor cells, EPCs) transplantation for rat middle cerebral artery occlusion model, observe the Bcl-2 and Bax expression levels. Explore BMSCs and EPCs transplantation in acute focal cerebral ischemia treatment effects and possible mechanisms. Methods SD rats suture method model of middle cerebral artery occlusion (MCAO) (n = 100), based on completely randomly divided into sham group (A group), ischemic natural recovery group (B), a single BMSCs transplantation group (C group), single transplanted EPCs group (D group) and BMSCs EPCs transplantation group (E). A group of anesthesia, only separated from the internal carotid artery, that is not treated skin was sutured, B group was MCAO rat model group; C, D, E transplantation ischemia 2h (hours) followed by reperfusion 24h, respectively, through the tail vein injection of 2 × 10 ~ 6 BMSCs, 2 × 10 ~ 6EPCs, an equal volume of 2 × 106BMSCs and 2 × 106EPCs fluid in single transplanted BMSCs group, single transplanted EPCs group and BMSCs EPCs transplantation group. 7 days after transplantation, were evaluated neurobehavioral score, HE staining of cell morphology, measuring protein bcl-2 and bax expression. Results 1. Neurobehavioral rating: A group 0, B group 3.92 ± 0.31, C group, 2.67 ± 0.43, D group, 3.01 ± 0.45 points, E group, 1.92 ± 0.25 points. Group B was significantly higher than that in group C, D group, with a significant difference (P lt; 0.05), while E and C group, D group was significantly different (P lt; 0.05). 2. Bcl-2 immunohistochemical staining: Bcl-2 expression in group A, 8.69 ± 10.14, B group 12.59 ± 4.26, C group, 33.37 ± 6.51, D group, 22.28 ± 7.18, E group 45.67 ± 5.02. In group E was the highest compared with the other groups were statistically significant (P lt; 0.05), C group, D group compared with group B were significantly different (P lt; 0.05), C, D group phase statistically significant difference compared with (P lt; 0.05). 3. Bax immunohistochemical staining: Bax expression in group A, 7.15 ± 8.50, B group 39.89 ± 8.46, C group, 21.32 ± 3.91, D group, 28.57 ± 4.67, E group 10.49 ± 7.24. Strongest expression in group B, C group, D group followed, E group was the lowest. B and C, D group compared with the significant difference (P lt; 0.05), E group and C, D group compared with the significant difference (P lt; 0.05). C, D group compared with the statistically significant difference (P lt; 0.05). 4 HE staining of brain tissue shows: B group organization edema, nerve cells increase in size, vacuolar degeneration, cell gap increases; E group more than the number of nerve cells, abundant cytoplasm, cell damage levels in each group of the most Light, low magnification seen scattered in small malacia remaining structure is relatively good. Conclusions 1. Mesenchymal stem cells and endothelial progenitor cell transplantation for the treatment of ischemic brain injury can promote recovery of neurological function, transplantation better than single cell transplantation. 2 mesenchymal stem cells and endothelial progenitor cell transplantation in the treatment of ischemic brain injury may be one of the mechanisms by upregulating the expression of Bcl-2, Bax protein expression decreased, reducing neuronal apoptosis.
|