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The Study on Mutation Rules of Oncidium (Onc. Gower Ramsey "Golden No.3") Industrialized Seedling Cultivating

Author: LiJiangYu
Tutor: MoRao
School: Hainan University
Course: Crop Genetics and Breeding
Keywords: Oncidium Factory production Variation ISSR detection
CLC: S682.31
Type: Master's thesis
Year: 2011
Downloads: 41
Quote: 0
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Abstract


With Oncidium industry booming industrial production production Oncidium become an important source of seed . However, in the factory , the offspring produced by a clonal variation is a very common phenomenon, and factory production is intended to provide the same excellent quality seedlings , how to control the mutation rate of clonal progeny of Man Oncidium factory production has a very important significance. To control somaclonal variation , we must fully understand the explant morphogenesis , and mutate in the process of the law, in order to better control their mutation rate , not only in theory but in practice there significance. I passed on industrial production in Oncidium Variation of the following results : 1 for the first time the system has been Oncidium orchid stem explants differentiate three ways: ( 1 ) organ type ; ( 2 ) type of organogenesis ; ( 3 ) explants into PLBs . 2 through systematic follow-up observations , to understand Oncidium sword from explants regenerated morphogenesis in the process. Explants through adventitious buds ( organ type ) and callus into shoots ( organogenesis type ) and PLBs developmental pathways ultimately form a complete plant . 3 pairs of factory production of PLBs and Regeneration Research variation types , classification of PLBs five types of variation and plant regeneration of the nine types of variation . 4 established a factory production Oncidium variation in seedling screening system. In industrial production , the first to control subculture times in less than ten generations , followed by early culling variation types of PLBs , combined again excluding variation seedlings, seedling ultimately control the mutation rate and improve the quality of seedling production , reduce production costs. 5 created by ISSR molecular marker Oncidium variation in seedling factory production testing technology , and initially learned in Oncidium Variation in factory production . Through different culture factors on PLBs variation studies have shown that exogenous additives insufficient or excessive circumstances and subculture generations will be more than ten times as high as 11.6% of the mutation rate .

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CLC: > Agricultural Sciences > Gardening > Ornamental Horticulture ( flowers and ornamental trees) > Perennial Flowers > Other Flowers > Orchids
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