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Detection and Isolation Methods for Plant Quarantine Pathogens

Author: ShangLinLin
Tutor: XuDianSheng；YiJianPing
School: East China University of Science and Technology
Course: Biochemical Engineering
Keywords: Amylovora bacteria Watermelon bacterial fruit blotch pathogen Rape Phytophthora Separate Detect
CLC: S432.4
Type: Master's thesis
Year: 2011
Downloads: 59
Quote: 0
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Abstract


For the study of the phytosanitary pathogens separation and detection technology has been an important research content of the countries in the agricultural sector as well as port quarantine workers . China 's accession to WTO trade relations with other countries in the world closer , fruit plants, seedlings , seeds , and other commodities trading more frequently , so the entry plant quarantine pathogens separation and detection become more important , China's ecological system security has important implications . In this study the author of three important plant quarantine pathogen separation and detection technology . The main contents include : 1 , widely used PCR assay cherry fruit amylovora bacteria detection using molecular biology methods of entry amylovora bacteria cherry fruit carrying detection . Comparison of conventional PCR, nested PCR , three real-time PCR detection of nine kinds of primers and probes the actual detect the effect of cherry fruit samples amylovora bacteria . Found for the cherry fruit samples amylovora bacteria detects Suitable PCR primers and methods of detecting . 2 , watermelon bacterial fruit blotch pathogen immunomagnetic separation polyclonal antibody preparation of watermelon bacterial fruit blotch pathogen immunomagnetic bead technology used in enrichment and separation of watermelon bacterial fruit blotch pathogen . Immunomagnetic separation process by multi-resistant and improved isolation medium , and ultimately achieve the purpose of watermelon bacterial fruit blotch pathogen isolated from the the low carrier watermelon seed samples . 3 , the rapeseed blackleg Pathogen isolation and detection by colony and spore morphology , PCR detection of pathogenic detection test entering the country rapeseed samples isolated from two fungal isolates were identified as rapeseed blackleg disease two pathogens Leptosphaeria maculans and Leptosphaeria biglobosa. The design and synthesis of Leptosphaeria maculans conventional PCR analysis using primers lmb3 / 4 , and real-time PCR detection the primers used 1mb3/R2 and probe Probe-M and eventually prove the design of primers and probes can be used for the specific detection of Leptosphaeria maculans .

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CLC: > Agricultural Sciences > Plant Protection > Pest and Disease Control > Plant diseases and their prevention > Transgression ( pass ) an infectious agent harmful
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