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The Research of Anti-proliferation and Induction of Apoptosis of Bufalin on Human Gastric Cell Line MGC803 in Vitro

Author: WangZuo
Tutor: ZhuZhiTu
School: Liaoning Medical
Course: Oncology
Keywords: Bufalin Gastric cancer Apoptosis Livin
CLC: R735.2
Type: Master's thesis
Year: 2011
Downloads: 44
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Abstract


ObjectiveTo assess the inhibition of proliferation and the induction of apoptosis of bufalin on human gastric (MGC803) cells.we investigated the effect of Livin and Caspase-3 and the possible mechanism in bufalin-induced gastric cancer MGC803 cells apoptosis.Methods(1)The effect of bufalin on MGC803 cell proliferation was measured using MTT assay.(2)Morphological analysis was performed by cytospin preparation with Wright-Giemsa stain and the slides were observed under a light microscope.(3)Cell cycle phase distribution and hypodiploid DNA was determined by flow cytometry with PI.(4)Expression of Livin、Caspase-3 was analyzed by Western blot.Results(1)MTT assay showed that bufalin inhibited MGC803 cell proliferation in time and dose-dependent manner.The inhibiting concentration of 50% cell growth (IC50) at 24h,48h and 72h was 105.25±6.27nmol/L、47.92±4.32nmol/L and 16.52±2.81nmol/L,respectively.(2)Apoptosis of MGC803 cells could be efficiently induced by bufalin,apoptotic bodies and sub-G1 peak (representing apoptosis) emerged when the cells were treated with different concentrations.The percentage of apoptosis cells were (2.10±0.51)% ,(3.71±1.53)% and (20.83±2.72)% after the cells exposed to 20nmol/L,40nmol/L and 80nmol/L bufalin for 24h,(P<0.01)compare with the untreated control group.(3)The expression of Livin protein was downregulated(P<0.01) and Caspase-3 protein was activated during bufalin-induced apoptosis in MGC803 cells.ConclusionsBufalin suppresses the growth of MGC803 cells and induces apoptosis, inhibition of Livin and activation of Caspase-3 may be involved in its mechanism.

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CLC: > Medicine, health > Oncology > Gastrointestinal Cancer > Gastric neoplasms
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