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Black tiger palm bacteria generic tooth fungus jujube meat Alice scales (Sarcodon imbricatus (L.ex Fr.) Karst.), Is an edible and medicinal fungi belonging Basidiomycetes Polyporaceae Polyporus, is Yunnan Guizhou and Tibet region's unique natural black fungus, with high tonic value. Black tiger palm current research focuses primarily on bacteria black tiger palm crude polysaccharide antibacterial activity and in vivo anti-tumor effect, purified polysaccharide structure studies and in vitro inhibition of tumor cells has not been reported differences in study. The topics to black tiger palm bacteria as raw material for the black tiger palm dried bacteria in nutrients, black tiger palm polysaccharide Purification, characterization and antitumor activity of the research, the main findings are as follows: black tiger palm bacteria dried in the protein content of 12.66%, a fat content of 2.57%, ash 9.56%, a water content of 7.83%, a total of 67% carbohydrate. Major elements phosphorus and potassium content up to approximately 20000 mg / kg, magnesium content was 751.55 mg / kg; trace elements iron as 318.07 mg / kg, zinc was 116.77 mg / kg, manganese, copper, boron also have some content. Black tiger palm bacteria CPC contains 17 kinds of amino acids, the total content of 9888.83 mg/100 g, which accounted for 33.77% of essential amino acids. Extracted with hot water black tiger palm polysaccharide, after alcohol precipitation, in addition to protein, bleaching get black tiger palm polysaccharide SIP, SIP through DE-52 ion-exchange chromatography, Sepharose CL-6B gel column chromatography to give six purified polysaccharide: SIP-1-1, SIP-1-2, SIP-2-1, SIP-2-2, SIP-3-1 and SIP-3-2, GPC, and Sepharose CL-6B gel column Chromatography polysaccharide samples were identified six single component, weight-average molecular weight of 2.12 × 10 ~ 5 Da, 1.21 × 10 ~ 4 Da, 4.55 × 10 ~ 5 Da, 1.23 × 10 ~ 4 Da, 7.45 × 10 ~ 5 Da and 1.19 × 10 ~ 4 Da. IR, UV, GC analysis showed that purified bacterial polysaccharide black tiger palm SIP-1-1, SIP-1-2, SIP-2-1, SIP-2-2, SIP-3-1 and SIP-3-2 contain nucleic acids and proteins, contain 3418 ~ 3405 cm-1 at the OH stretching vibration, 2932 ~ 2927 cm-1 at the CH stretching vibration and 1430 ~ 1200 cm-1 at a carboxyl group stretching vibrations, which are polysaccharide characteristic infrared absorption. Monosaccharide composition analysis: SIP contains fucose, mannose, glucose and galactose, the molar ratio of: fucose: mannose: glucose: galactose = 3.7:1:20.17:12.49; SIP-1 -1, SIP-2-1 and SIP-3-1 containing only glucose; SIP-1-2 and the SIP-2-2 contain fucose, glucose, galactose, mannose, galactose-based; SIP -3-2 containing glucose and galactose. Use of human hepatoma Hep G2 cells, human ovarian cancer HO-8910 and BT-474 human breast cancer cells in vitro three kinds of tumor cell proliferation inhibition assay for black tiger palm polysaccharide SIP, SIP-1-1, SIP-1-2, SIP-2-1, SIP-2-2, SIP-3-1 and SIP-3-2 anti-tumor activity screening, the majority of black tiger palm polysaccharide on three kinds of tumor cells have a strong inhibitory effect, and dose-dependent manner; wherein the high molecular weight polysaccharide SIP-2-1 and SIP-1-1 inhibition of the three most obvious, SIP-1-1 at high concentrations in human breast cancer cells was inhibited by 36.05%, not and small molecules purified polysaccharide polysaccharide SIP SIP-3-2 inhibition rate is low. Periodate oxidation, Smith degradation and NMR results showed that the black tiger palm polysaccharide SIP-2-2 is mainly configuration, small part of the β configuration, C-6 and C-2, C-3 and C-4 has been substituted in its sugar chain is 位 1 → 3, 1 → 4-bit and bit 1 → 4,6-bonded galactose residue as a main chain, with 1 → 2 bits and 1 → 3 bond co-fucose, glucose, mannose branched heteropolysaccharide.
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