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Objective This study astragalus (Astragalus membranaceus injection) on rat hepatic ischemia-reperfusion injury in rats, and with ischemic preconditioning (Ischemia preconditioning, IP) to compare effects and to explore its mechanism. Methods Male SD (Spragu-Dawley) rats were 96, were randomly divided into four groups, namely, sham-operated (Sham operation, Sham) group, ischemia-reperfusion (Ischemia-reperfusion, IR) group, ischemic preconditioning ( Ischemia preconditioning, IP) group, Astragalus (Astragalus membranaceus, AM) groups and at the time of reperfusion (1h, 3h, 6h, 24h) is divided into four time points, each group at each time point was 6. Preparation of 70% in rat liver ischemia-reperfusion model (Pringle's law), that is to take rat abdominal incision, isolated hepatic ligament, with no damage to the liver artery clip and left middle lobe artery, portal vein and bile duct be blocked artery clip removed after 40min ischemia restore perfusion. Ischemic preconditioning in experimental liver middle and left before the first leaf artery, portal vein and biliary be blocked ischemia 5min, then open flow 5min, repeated three times. Astragalus pretreatment is administered by 12ml/kg (concentration 2g/ml), open from tail vein injection 5min before; Sham group, IR group, IP group 5min before laparotomy were from the tail vein at 12ml/kg slow injection of saline. Each reperfusion 1h, 3h, 6h, 24h drawn the four time points, the heart chamber prick After centrifugation, using automatic biochemical analyzer measurements of serum alanine aminotransferase (Alanine aminotransferase, ALT), aspartate acid transaminase (Aspartate aminotransferase, AST), lactate dehydrogenase (Lactate dehydrogenase, LDH) levels; take the left hepatic tissue fixed position, colorimetric determination of liver tissue myeloperoxidase (Myeloperoxidase, MPO) levels; immunohistochemistry method (SP method) liver tissue of tumor necrosis factor-α (Tumor necrosis factor-α, TNF-α), interleukin -10 (Interleukin-10, IL-10) expression; and light microscopy of liver tissue injury and electron microscopy ultrastructural changes in rat liver cells. The results of liver enzyme changes: IR group, IP group, AM group, ALT, AST, LDH and MPO levels were significantly higher than the Sham group (P lt; 0.01). Compared with IR group, IP group, AM group at each time point values ??were decreased (P lt; 0.05). And IP group, AM group, a lower value at each time point (P lt; 0.05). Immunohistochemical TNF-α, IL-10 expression: IR group, IP group, AM group, liver tissue TNF-α, IL-10 positive cells expressed high rate than the Sham group (P lt; 0.05); with IR group comparison, IP group, AM group decreased expression of TNF-α, and increased expression of IL-10 (P lt; 0.05); and IP group, AM group decreased expression of TNF-α, IL-10 expression increased (P lt ; 0.05). IL-10 and TNF-α, MPO change into a negative correlation between indicators (P lt; 0.01). Light microscopy of liver histological changes: Sham group, normal liver cells, hepatic cord arrangement rules; IR group lobule central vein congestion obvious, there are a lot of sinusoidal accumulation of inflammatory cells; IP group lobule central vein and hepatic sinusoidal congestion lighter, few inflammatory cells; AM group within the lobule and sinus congestion is slight, normal lobular architecture. Electron microscopy of liver cell ultrastructure: Sham group arrangement rules mitochondria, the nucleus membrane dual structure clear cytoplasm endoplasmic reticulum arrangement rules; IR group markedly swollen mitochondria expansion cristae, nuclear membrane disappears nucleus, endoplasmic reticulum ; IP group mitochondria slight swelling, no significant expansion or vacuolar degeneration; AM small set slightly swollen mitochondria, membrane structure is very clear that a slight expansion of the endoplasmic reticulum. Thus, IR group damage is obvious, IP group and the AM group damage lighter than IR group, AM group, lighter, and Sham group morphologically normal liver. Conclusion hepatic ischemia-reperfusion injury of liver damage was, astragalus and ischemic preconditioning can inhibit the liver tissue TNF-α expression and promote the expression of IL-10 to reduce ischemia-reperfusion injury in the liver, and the former later by effect.
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